Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2358-2362. doi: 10.1099/ijs.0.028829-0. Epub 2010 Oct 29.
Two novel actinomycete strains, designated KB2-4(T) and KB9-2, were isolated from soil samples. Both isolates formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid; the whole-cell sugars were galactose, glucose, mannose, rhamnose, ribose and xylose. The diagnostic phospholipid was phosphatidylethanolamine. The predominant menaquinones were MK-9(H(8)) and MK-9(H(6)). Mycolic acids were not detected. The predominant cellular fatty acids (>20 %) were iso-C(16 : 0) and anteiso-C(17 : 0). For both strains, the G+C content of the genomic DNA was about 72 mol%. The morphological and chemotaxonomic characteristics of the two strains were typical of members of the genus Dactylosporangium. Phylogenetic analysis using 16S rRNA gene sequences also indicated that these strains should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium salmoneum NRRL B-16294(T) (99.1 % 16S rRNA gene sequence similarity). DNA-DNA relatedness and some physiological and biochemical properties indicated that the isolates could be readily distinguished from their closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, the isolates represent a novel species, for which the name Dactylosporangium tropicum sp. nov. is proposed. The type strain is strain KB2-4(T) ( = BCC 34760(T) = JCM 15673(T)).
从土壤样本中分离到两株新型放线菌菌株，分别命名为 KB2-4(T) 和 KB9-2。这两株菌都在短的孢囊梗上形成指状的孢囊，孢囊梗直接从基质菌丝上生出。细胞壁肽聚糖含有谷氨酸、甘氨酸、丙氨酸和内消旋二氨基庚二酸；全细胞糖为半乳糖、葡萄糖、甘露糖、鼠李糖、核糖和木糖。特征性磷脂为磷脂酰乙醇胺。主要的menaquinones 为 MK-9(H(8)) 和 MK-9(H(6))。未检测到分枝菌酸。主要的细胞脂肪酸 (>20 %)为 iso-C(16 : 0)和 anteiso-C(17 : 0)。两株菌的基因组 DNA G+C 含量约为 72 mol%。这两株菌的形态学和化学分类特征均典型地属于游动放线菌属。16S rRNA 基因序列的系统发育分析也表明，这些菌株应归入游动放线菌属，与 Dactylosporangium salmoneum NRRL B-16294(T)（99.1 % 16S rRNA 基因序列相似性）最为接近。DNA-DNA 同源性和一些生理生化特性表明，这些分离株很容易与它们最接近的系统发育亲缘种区分开来。基于这些表型和基因型数据，这些分离株代表了一个新的种，命名为热带游动放线菌（Dactylosporangium tropicum sp. nov.）。模式菌株为 KB2-4(T) 株（ = BCC 34760(T) = JCM 15673(T)）。
