Liu Chongxi, Guan Xuejiao, Jin Pinjiao, Li Jinmeng, Li Yao, Li Lianjie, Zhou Ying, Shen Yue, Wang Xiangjing, Xiang Wensheng
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Jul;108(1):191-9. doi: 10.1007/s10482-015-0478-3. Epub 2015 May 20.
A novel actinobacterium, designated strain NEAU-TPG4(T) was isolated from rhizosphere soil of Pinus koraiensis Sieb. et Zucc. collected from Luobei, Heilongjiang Province, north China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-TPG4(T) were consistent with the description of the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan consisted of meso- and 3-hydroxy-diaminopimelic acids; arabinose, xylose and glucose were found as whole-cell sugars. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were identified as iso-C16:0, anteiso-C15:0, anteiso-C17:0 and C18:0. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relatives were Dactylosporangium maewongense Japan Collection of Microorganism (JCM) 15933(T) (99.6 %), Dactylosporangium siamense NBRC 106093(T) (99.6 %), Dactylosporangium aurantiacum JCM 3083(T) (99.5 %) and Dactylosporangium luteum JCM 17685(T) (99.5 %). However, the low level of DNA-DNA relatedness and several cultural and physiological characteristics allowed the strain to be distinguished from its closest relatives. Thus, it is proposed that strain NEAU-TPG4(T) represents a novel Dactylosporangium species. Dactylosporangium cerinum sp. nov. The type strain of D. cerinum is NEAU-TPG4(T) (=CGMCC 4.7152(T) = DSM 46712(T)).
从中国北方黑龙江省萝北县采集的红松（Pinus koraiensis Sieb. et Zucc.）根际土壤中分离出一株新型放线菌，命名为菌株NEAU-TPG4(T)，并采用多相分类法对其进行了鉴定。菌株NEAU-TPG4(T)的形态和化学分类学特征与指孢囊菌属（Dactylosporangium）的描述一致。该菌株在直接从基质菌丝长出的短孢囊梗上形成指状孢囊。细胞壁肽聚糖由内消旋二氨基庚二酸和3-羟基二氨基庚二酸组成；全细胞糖中发现有阿拉伯糖、木糖和葡萄糖。磷脂图谱包含双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰肌醇。主要的甲基萘醌被鉴定为MK-9(H8)和MK-9(H6)。主要的细胞脂肪酸被鉴定为异-C16:0、anteiso-C15:0、anteiso-C17:0和C18:0。使用16S rRNA基因序列进行的系统发育分析也表明，该菌株应归类于指孢囊菌属，并显示其最密切的亲缘关系是Mae Wong指孢囊菌（Dactylosporangium maewongense）日本微生物保藏中心（JCM）15933(T)（99.6%）、暹罗指孢囊菌（Dactylosporangium siamense）日本国立技术与评价研究所生物资源中心（NBRC）106093(T)（99.6%）、橙色指孢囊菌（Dactylosporangium aurantiacum）JCM 3083(T)（99.5%）和黄色指孢囊菌（Dactylosporangium luteum）JCM 17685(T)（99.5%）。然而，低水平的DNA-DNA相关性以及一些培养和生理特征使该菌株能够与其最密切的亲缘关系区分开来。因此，建议菌株NEAU-TPG4(T)代表一个新的指孢囊菌物种。蜡黄指孢囊菌（Dactylosporangium cerinum）新种。蜡黄指孢囊菌的模式菌株是NEAU-TPG4(T)（=中国普通微生物菌种保藏管理中心（CGMCC）4.7152(T) = 德国微生物保藏中心（DSM）46712(T)）。
