重组大肠杆菌中戊二酸的生产。
Production of glutaconic acid in a recombinant Escherichia coli strain.
机构信息
Fachbereich Biologie, Philipps-Universität, Marburg, Germany.
出版信息
Appl Environ Microbiol. 2011 Jan;77(1):320-2. doi: 10.1128/AEM.02049-10. Epub 2010 Oct 29.
Abstract
The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutaryl-coenzyme A (CoA), and (E)-glutaconyl-CoA to yield 2.7 ± 0.2 mM (E)-glutaconate in the medium.
摘要
当在复杂培养基中进行厌氧生长时，来自谷氨酸发酵细菌的 6 种酶的基因的组装将大肠杆菌转化为戊二酸生产菌。新的厌氧途径从一般代谢中的 2-酮戊二酸开始，然后经过（R）-2-羟基戊二酸、（R）-2-羟基戊二酰辅酶 A（CoA）和（E）-戊二酰辅酶 A，在培养基中产生 2.7±0.2mM 的（E）-戊二酸。
相似文献
1
Production of glutaconic acid in a recombinant Escherichia coli strain.重组大肠杆菌中戊二酸的生产。
Appl Environ Microbiol. 2011 Jan;77(1):320-2. doi: 10.1128/AEM.02049-10. Epub 2010 Oct 29.
2
Glutaconate CoA-transferase from Acidaminococcus fermentans.来自发酵氨基酸球菌的戊二酸盐辅酶A转移酶。
Eur J Biochem. 1981 Aug;118(2):315-21. doi: 10.1111/j.1432-1033.1981.tb06404.x.
3
Substrate specificity of 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum: toward a bio-based production of adipic acid.梭菌属 2-羟基戊二酰辅酶 A 脱水酶的底物特异性：朝着生物基己二酸生产的方向。
Biochemistry. 2011 May 3;50(17):3540-50. doi: 10.1021/bi1020056. Epub 2011 Apr 5.
4
Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans.编码戊二酸盐辅酶A转移酶的两个基因在发酵氨基酸球菌中羟基戊二酸操纵子起始处的定位。
Eur J Biochem. 1994 Nov 15;226(1):41-51. doi: 10.1111/j.1432-1033.1994.tb20024.x.
5
Synthesis and properties of (R)-2-hydroxyglutaryl-1-CoA. (R)-2-hydroxyglutaryl-5-CoA, an erroneous product of glutaconate CoA-transferase.（R）-2-羟基戊二酰辅酶A的合成与性质。（R）-2-羟基戊二酰辅酶A，戊烯二酸辅酶A转移酶的错误产物。
Biol Chem Hoppe Seyler. 1991 May;372(5):319-24. doi: 10.1515/bchm3.1991.372.1.319.
6
Engineering Escherichia coli for efficient cellobiose utilization.工程化大肠杆菌以提高纤维二糖利用率。
Appl Microbiol Biotechnol. 2011 Oct;92(1):125-32. doi: 10.1007/s00253-011-3434-9. Epub 2011 Jun 29.
7
Driving forces enable high-titer anaerobic 1-butanol synthesis in Escherichia coli.在大肠杆菌中，驱动因素使高浓度厌氧 1-丁醇的合成成为可能。
Appl Environ Microbiol. 2011 May;77(9):2905-15. doi: 10.1128/AEM.03034-10. Epub 2011 Mar 11.
8
Bioconversion of ethyl (R)-4-cyano-3-hydroxybutyate into (R)-ethyl-3-hydroxyglutarate via an indirect pathway by Rhodococcus boritolerans.经土壤红球菌（Rhodococcus boritolerans）间接途径生物转化（R）-4-氰基-3-羟基丁酸乙酯为（R）-乙基-3-羟基戊二酸酯。
Biotechnol Lett. 2012 May;34(5):901-5. doi: 10.1007/s10529-012-0846-2. Epub 2012 Jan 20.
9
The reversible dehydration of (R)-2-hydroxyglutarate to (E)-glutaconate.(R)-2-羟基戊二酸向(E)-戊烯二酸的可逆脱水反应。
Eur J Biochem. 1980 May;106(2):439-47. doi: 10.1111/j.1432-1033.1980.tb04590.x.
10
High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli.通过基于化学计量学最大值在大肠杆菌中策略性地调控多种代谢途径实现高产厌氧琥珀酸生产。
Microb Cell Fact. 2016 Aug 12;15(1):141. doi: 10.1186/s12934-016-0536-1.
引用本文的文献
1
A Synthetic Pathway for the Production of Benzylsuccinate in .在. 中生产苯丁烯二酸的合成途径。
Molecules. 2024 Jan 15;29(2):415. doi: 10.3390/molecules29020415.
2
Molecular mechanisms of microbiome modulation by the eukaryotic secondary metabolite azelaic acid.真核次生代谢产物壬二酸调控微生物组的分子机制。
Elife. 2024 Jan 8;12:RP88525. doi: 10.7554/eLife.88525.
3
Influence of grape consumption on the human microbiome.葡萄消费对人类微生物组的影响。
Sci Rep. 2023 May 12;13(1):7706. doi: 10.1038/s41598-023-34813-5.
4
Deep learning driven biosynthetic pathways navigation for natural products with BioNavi-NP.深度学习驱动的生物合成途径导航用于天然产物的 BioNavi-NP。
Nat Commun. 2022 Jun 10;13(1):3342. doi: 10.1038/s41467-022-30970-9.
5
Alpha-ketoglutarate utilization in Saccharomyces cerevisiae: transport, compartmentation and catabolism.α-酮戊二酸在酿酒酵母中的利用：运输、区隔化和分解代谢。
Sci Rep. 2020 Jul 30;10(1):12838. doi: 10.1038/s41598-020-69178-6.
6
Advances in bio-based production of dicarboxylic acids longer than C4.C4以上二元羧酸生物基生产的进展
Eng Life Sci. 2018 Jul 24;18(9):668-681. doi: 10.1002/elsc.201800023. eCollection 2018 Sep.
7
Discovery and implementation of a novel pathway for -butanol production via 2-oxoglutarate.通过2-氧代戊二酸发现并实现了一条生产丁醇的新途径。
Biotechnol Biofuels. 2019 Sep 30;12:230. doi: 10.1186/s13068-019-1565-x. eCollection 2019.
8
Syntrophus aciditrophicus uses the same enzymes in a reversible manner to degrade and synthesize aromatic and alicyclic acids.产琥珀酸丝氨酸弧菌以可逆的方式使用相同的酶来降解和合成芳香族和脂环族酸。
Environ Microbiol. 2019 May;21(5):1833-1846. doi: 10.1111/1462-2920.14601.
9
Exploring D-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway.通过魏姆伯格途径探索酿酒酵母中的D-木糖氧化作用。
AMB Express. 2018 Mar 5;8(1):33. doi: 10.1186/s13568-018-0564-9.
10
Engineering nonphosphorylative metabolism to generate lignocellulose-derived products.工程非磷酸化代谢以生成木质纤维素衍生产品。
Nat Chem Biol. 2016 Apr;12(4):247-53. doi: 10.1038/nchembio.2020. Epub 2016 Feb 8.
本文引用的文献
1
Structural basis for promoting and preventing decarboxylation in glutaryl-coenzyme a dehydrogenases.促进和抑制戊二酰辅酶 A 脱氢酶脱羧作用的结构基础。
Biochemistry. 2010 Jun 29;49(25):5350-7. doi: 10.1021/bi100317m.
2
Systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane.在谷氨酸棒杆菌中进行全系统代谢途径工程以生物合成二氨基戊烷。
Metab Eng. 2010 Jul;12(4):341-51. doi: 10.1016/j.ymben.2010.03.005. Epub 2010 Apr 8.
3
On the thermodynamic equilibrium between (R)-2-hydroxyacyl-CoA and 2-enoyl-CoA.（R）-2-羟酰基辅酶 A 与 2-烯酰基辅酶 A 之间的热力学平衡。
FEBS J. 2010 Apr;277(7):1738-46. doi: 10.1111/j.1742-4658.2010.07597.x. Epub 2010 Feb 23.
4
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli.大肠杆菌中的绝对代谢物浓度及隐含的酶活性位点占有率
Nat Chem Biol. 2009 Aug;5(8):593-9. doi: 10.1038/nchembio.186. Epub 2009 Jun 28.
5
Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.通过巴豆酰辅酶A羧化酶/还原酶由C2单位合成C5-二羧酸：乙基丙二酰辅酶A途径。
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10631-6. doi: 10.1073/pnas.0702791104. Epub 2007 Jun 4.
6
Structural basis for stereo-specific catalysis in NAD(+)-dependent (R)-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans.来自发酵氨基酸球菌的依赖NAD⁺的（R）-2-羟基戊二酸脱氢酶立体特异性催化的结构基础。
FEBS J. 2005 Jan;272(1):269-81. doi: 10.1111/j.1432-1033.2004.04417.x.
7
On the mechanism of action of the antifungal agent propionate.关于抗真菌剂丙酸盐的作用机制
Eur J Biochem. 2004 Aug;271(15):3227-41. doi: 10.1111/j.1432-1033.2004.04255.x.
8
Unusual enzymes involved in five pathways of glutamate fermentation.参与谷氨酸发酵五条途径的特殊酶。
Appl Microbiol Biotechnol. 2001 Oct;57(3):263-73. doi: 10.1007/s002530100773.
9
The iron-sulfur clusters in 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. Biochemical and spectroscopic investigations.来自发酵氨基酸球菌的2-羟基戊二酰辅酶A脱水酶中的铁硫簇。生化与光谱研究。
Eur J Biochem. 2000 Dec;267(24):7082-93. doi: 10.1046/j.1432-1327.2000.01809.x.
10
2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum.
Eur J Biochem. 1999 Oct 1;265(1):404-14. doi: 10.1046/j.1432-1327.1999.00748.x.
Zotero 插件