Department of Dermatology, University Hospital Jena, Jena, Germany.
Contact Dermatitis. 2010 Dec;63(6):347-56. doi: 10.1111/j.1600-0536.2010.01777.x. Epub 2010 Oct 7.
Skin exposure to lipophilic irritants at workplaces is a risk factor for the development of occupational irritant hand dermatitis. Suitable lipophilic model irritants to be used in repeated irritation studies in volunteers are needed to gain a better understanding of the chemical-dependent mechanisms of skin irritation.
To investigate acute and cumulative irritation elicited by two new lipophilic model irritants, octane (CAS No. 111-65-9) and cumene (CAS No. 98-82-8), in stingers as compared with non-stingers.
Short-time occlusive and repetitive occlusive irritation patch tests were performed over 5 days with octane and cumene in two groups of healthy volunteers (n = 15 each) who were classified as stingers and non-stingers according to a preceding lactic acid stinging test. Acute and cumulative irritation was quantified using clinical assessments and bioengineering methods (laser Doppler flowmetry, transepidermal water loss, capacitance measurements, and skin colour reflectance).
Significantly stronger cumulative irritation was observed in stingers than in non-stingers for both irritants (visual scoring, stratum corneum hydration, and skin colour reflectance).
The preliminary results suggest the existence of a distinct sub-population with increased susceptibility to cumulative irritation induced by lipophilic irritants that can be identified by the lactic acid stinging test.
工作场所皮肤接触亲脂性刺激物是职业性刺激性接触性皮炎发展的一个风险因素。需要合适的亲脂性模型刺激物来进行志愿者的重复刺激研究，以便更好地了解皮肤刺激的化学依赖性机制。
研究两种新型亲脂性模型刺激物辛烷（CAS 号 111-65-9）和异丙苯（CAS 号 98-82-8）在蜇刺者和非蜇刺者中的急性和累积性刺激作用。
在两组健康志愿者（每组 15 人）中进行了为期 5 天的短时间封闭和重复封闭刺激斑贴试验，这些志愿者根据先前的乳酸刺痛试验被分类为蜇刺者和非蜇刺者。使用临床评估和生物工程方法（激光多普勒血流仪、经表皮水分流失、电容测量和皮肤颜色反射率）来量化急性和累积性刺激。
对于两种刺激物，蜇刺者的累积刺激明显强于非蜇刺者（视觉评分、角质层水合作用和皮肤颜色反射率）。
初步结果表明，存在一种特定的亚人群，他们对亲脂性刺激物引起的累积性刺激具有更高的敏感性，可以通过乳酸刺痛试验来识别。
