Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
J Ethnopharmacol. 2011 Jan 27;133(2):710-7. doi: 10.1016/j.jep.2010.10.047. Epub 2010 Oct 30.
For thousands of years antlers have been used in Asian countries to promote rapid healing, treat weight loss, slow growth in children, strengthen weak bones, and alleviate cold hands and feet.
The present study was performed to examine the effect of fermentation on the ability of antler to act as a stimulator of bone growth.
This study used pre-osteoblast MC3T3-E1 cells to examine factors related to bone growth, such as cell proliferation, production of alkaline phosphatase (ALP), and deposition of extracellular matrix proteins (e.g., collagens, osteonectin, bone sialoprotein (BSP)), via the treatment of non-fermented and fermented antler.
Antler fermentation using Cordyceps militaris was carried out at 25°C for seven days. The total content of sugar, sialic acid, and protein increased with fermentation time. Cell proliferation was greater in the fermented antler- (FA-) treated groups than in the NFA- (non-fermented antler-) treated groups, in which proliferation increased significantly up to 137% of the basal value. Significant increases in mRNA expression and ALP activity were found at FA concentrations of 50-100 μg/ml; at 100 μg/ml the activity had increased 119% compared to the control activity. For NFA and FA the expression levels of type I collagen mRNA significantly increased in a dose-dependent manner at all treatment doses. However, significant differences between the antler groups were not observed. Mineralization significantly increased by NFA and FA treatment to 183% and 241%, respectively, when compared to colostrum, as a positive control (165%).
Antler treatment increased the proliferation of osteoblasts and bone matrix proteins, such as type I collagen and BSP. Antler fermented with Cordyceps militaris showed enhanced activity, and its stimulatory effects on cell proliferation and ALP production were greater than those of NFA. We surmise that these increases in activity were related to increased sialic acid content. Therefore, the results of this study suggest that the physiological effects of antler, including bone growth, may be increased through the fermentation process.
几千年来，鹿角一直被亚洲国家用于促进快速愈合、治疗体重减轻、儿童生长缓慢、增强虚弱的骨骼以及缓解手脚冰冷。
本研究旨在研究发酵对鹿角作为骨生长刺激物的能力的影响。
本研究使用前成骨细胞 MC3T3-E1 细胞，通过非发酵和发酵鹿角处理，检查与骨生长相关的因素，如细胞增殖、碱性磷酸酶（ALP）的产生以及细胞外基质蛋白（如胶原蛋白、骨唾液蛋白（BSP））的沉积。
采用蛹虫草对鹿角进行发酵，在 25°C 下发酵七天。随着发酵时间的增加，糖、唾液酸和蛋白质的总量增加。发酵鹿角（FA）处理组的细胞增殖大于非发酵鹿角（NFA）处理组，增殖增加了高达 137%的基础值。在 FA 浓度为 50-100μg/ml 时，发现 mRNA 表达和 ALP 活性显著增加；在 100μg/ml 时，活性比对照活性增加了 119%。对于 NFA 和 FA，在所有处理剂量下，I 型胶原蛋白 mRNA 的表达水平均呈剂量依赖性显著增加。然而，鹿角组之间没有观察到显著差异。与初乳（阳性对照，165%）相比，NFA 和 FA 处理均显著增加矿化，分别增加到 183%和 241%。
鹿角处理增加了成骨细胞和骨基质蛋白（如 I 型胶原蛋白和 BSP）的增殖。用蛹虫草发酵的鹿角显示出增强的活性，其对细胞增殖和 ALP 产生的刺激作用大于 NFA。我们推测这些活性的增加与唾液酸含量的增加有关。因此，本研究的结果表明，鹿角的生理作用，包括骨生长，可能通过发酵过程得到增强。
