Department of Clinical Neurobiology, IZN, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Neuron. 2010 Nov 4;68(3):557-69. doi: 10.1016/j.neuron.2010.09.017.
Activity of parvalbumin-positive hippocampal interneurons is critical for network synchronization but the receptors involved therein have remained largely unknown. Here we report network and behavioral deficits in mice with selective ablation of NMDA receptors in parvalbumin-positive interneurons (NR1(PVCre-/-)). Recordings of local field potentials and unitary neuronal activity in the hippocampal CA1 area revealed altered theta oscillations (5-10 Hz) in freely behaving NR1(PVCre-/-) mice. Moreover, in contrast to controls, in NR1(PVCre-/-) mice the remaining theta rhythm was abolished by the administration of atropine. Gamma oscillations (35-85 Hz) were increased and less modulated by the concurrent theta rhythm in the mutant. Positional firing of pyramidal cells in NR1(PVCre-/-) mice was less spatially and temporally precise. Finally, NR1(PVCre-/-) mice exhibited impaired spatial working as well as spatial short- and long-term recognition memory but showed no deficits in open field exploratory activity and spatial reference learning.
钙蛋白酶阳性海马中间神经元的活动对于网络同步至关重要，但其中涉及的受体在很大程度上仍不清楚。本文报道了选择性敲除钙蛋白酶阳性中间神经元中 NMDA 受体（NR1(PVCre-/-)）的小鼠在网络和行为上的缺陷。在海马 CA1 区进行局部场电位和单位神经元活动记录显示，自由活动的 NR1(PVCre-/-) 小鼠的θ振荡（5-10 Hz）发生改变。此外，与对照组相比，在 NR1(PVCre-/-) 小鼠中，剩余的θ节律被阿托品给药消除。在突变体中，γ 振荡（35-85 Hz）增加，并且受同时的θ节律的调制减少。NR1(PVCre-/-) 小鼠中锥体神经元的位置放电在空间和时间上的精确性降低。最后，NR1(PVCre-/-) 小鼠表现出空间工作以及空间短期和长期识别记忆受损，但在开阔场探索活动和空间参照学习中没有缺陷。
