Departamento de Farmacologia, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-970, Curitiba-PR, Brazil.
Ecotoxicol Environ Saf. 2011 Mar;74(3):342-9. doi: 10.1016/j.ecoenv.2010.09.010. Epub 2010 Oct 30.
The use of the non-steroidal anti-inflammatory drugs such as dipyrone is so widespread that this drug and its metabolites have been detected in effluents and surface water. This study aimed to evaluate the potential toxic effects of dipyrone on the aquatic environment, using a native fish species, Rhamdia quelen. Fish were exposed to three concentrations of dipyrone, 0.5, 5 and 50 μg/L, in the water for 15 days, and hematological, biochemical, genetic and morphological biomarkers were evaluated. The glutathione S-transferase activity decreased in the highest concentration in relation to the control group. In addition, hematocrit, red blood cells and thrombocyte counts were decreased in all three exposed groups in relation to the control group. The comet assay showed DNA damage at the lowest concentration of dipyrone and significant kidney damage. Those results suggest that a constant exposure of aquatic organisms to dipyrone presents potential toxic effects.
非甾体抗炎药的使用非常广泛，如双氯芬酸，以至于这种药物及其代谢物已经在废水和地表水检测到。本研究旨在使用本地鱼类物种拉马氏脂鲤（Rhamdia quelen）评估双氯芬酸对水生环境的潜在毒性影响。将鱼暴露于水中三种不同浓度的双氯芬酸（0.5、5 和 50μg/L）中 15 天，并评估了血液学、生化、遗传和形态学生物标志物。与对照组相比，最高浓度的谷胱甘肽 S-转移酶活性降低。此外，与对照组相比，所有三个暴露组的血细胞比容、红细胞和血小板计数均降低。彗星试验显示在最低浓度的双氯芬酸下存在 DNA 损伤，并对肾脏造成严重损害。这些结果表明，水生生物持续暴露于双氯芬酸可能具有潜在的毒性影响。
