Division of Infectious Diseases, Oregon Health and Science University, Portland, OR, USA.
Antimicrob Agents Chemother. 2011 Jan;55(1):382-4. doi: 10.1128/AAC.01259-10. Epub 2010 Nov 1.
Among the 7 most common UL97 mutations encountered in ganciclovir-resistant clinical cytomegalovirus isolates, the associated cyclopropavir cross-resistance varies from insignificant (L595S) to substantial (M460I and H520Q) as determined by recombinant phenotyping. Mutations M460I and H520Q were preferentially selected in vitro under cyclopropavir and conferred 12- to 20-fold increases in 50% effective concentration (EC(50)) values, while M460V, C592G, A594V, and C603W conferred 3- to 5-fold increases. Uncommon mutations M460T and C603R increased cyclopropavir EC(50)s by 8- to 10-fold.
在更昔洛韦耐药的临床巨细胞病毒分离株中遇到的 7 种最常见的 UL97 突变中，通过重组表型测定，相关的环丙沙星交叉耐药性从无显著意义（L595S）到显著（M460I 和 H520Q）不等。突变 M460I 和 H520Q 在环丙沙星的体外选择中被优先选择，并导致 50%有效浓度（EC（50））值增加 12-20 倍，而 M460V、C592G、A594V 和 C603W 则增加 3-5 倍。不常见的突变 M460T 和 C603R 将环丙沙星的 EC（50）增加了 8-10 倍。
