Reitamo S, Anttila H S, Didierjean L, Saurat J H
Department of Dermatology, Helsinki University Central Hospital, Finland.
Br J Dermatol. 1990 Mar;122(3):315-23. doi: 10.1111/j.1365-2133.1990.tb08279.x.
Bouin-fixed paraffin sections or acetone-fixed cryostat sections were labelled with the avidin-biotin complex (ABC) or peroxidase-antiperoxidase (PAP) method using three monoclonal antibodies (MAbs) and two polyclonal antisera to human recombinant interleukin I beta (IL-I beta) and three polyclonal antisera to human recombinant interleukin I alpha (IL-I alpha). In the secretory coil both IL-alpha and beta were detected in the clear, but not in the dark cells. Both luminal and basal cells of the coiled and straight ducts expressed IL-I alpha and beta, the IL-I labelling being more intense in the luminal cells. IL-I was not usually detected in the initial portion of the intraepidermal eccrine sweat duct, whereas intense labelling was seen in the upper part including through the stratum corneum. In skin biopsies of the palm, taken after exercise, there was only faint IL-I labelling of the secretory cells, whereas the luminal cells of the dermal ducts showed intense labelling for both IL-I alpha and beta. In the acrosyringium, exercise did not alter the pattern for IL-I alpha and beta, except that in the palm, some of the antibodies to IL-I beta produced a more intense immunolabelling of the acrosyringeal cells. This study identifies a distinct and similar distribution of the two forms of IL-I throughout the eccrine sweat-gland apparatus and indicates that part of the IL-I epidermal pool originates from the sweat.
使用针对人重组白细胞介素Iβ（IL-Iβ）的三种单克隆抗体（MAb）和两种多克隆抗血清以及针对人重组白细胞介素Iα（IL-Iα）的三种多克隆抗血清，采用抗生物素蛋白-生物素复合物（ABC）法或过氧化物酶-抗过氧化物酶（PAP）法对Bouin固定的石蜡切片或丙酮固定的低温恒温器切片进行标记。在分泌盘曲部，在明细胞中检测到了IL-α和IL-β，但在暗细胞中未检测到。盘曲导管和直导管的管腔细胞和基底细胞均表达IL-Iα和IL-β，管腔细胞中的IL-I标记更强。在表皮内小汗腺导管的起始部分通常检测不到IL-I，而在上部包括角质层均可见强烈标记。在运动后取自手掌的皮肤活检标本中，分泌细胞只有微弱的IL-I标记，而真皮导管的管腔细胞对IL-Iα和IL-β均显示强烈标记。在顶泌汗腺导管，运动并未改变IL-Iα和IL-β的分布模式，只是在手掌，一些针对IL-Iβ的抗体使顶泌汗腺导管细胞产生更强的免疫标记。本研究确定了两种形式的IL-I在整个小汗腺器官中的独特且相似的分布，并表明表皮中部分IL-I来源汗液。
