基于平面光子晶体波导的单链 DNA 检测。

Single-strand DNA detection using a planar photonic-crystal-waveguide-based sensor.

机构信息

Nanophotonics Technology Center, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.

出版信息

Opt Lett. 2010 Nov 1;35(21):3673-5. doi: 10.1364/OL.35.003673.

DOI:10.1364/OL.35.003673

Abstract

We report an experimental demonstration of single-strand DNA (ssDNA) detection at room temperature using a photonic-crystal-waveguide-based optical sensor. The sensor surface was previously biofunctionalized with ssDNA probes to be used as specific target receptors. Our experiments showed that it is possible to detect these hybridization events using planar photonic-crystal structures, reaching an estimated detection limit as low as 19.8 nM for the detection of the complementary DNA strand.

摘要

我们报告了一种使用基于光子晶体波导的光学传感器在室温下进行单链 DNA（ssDNA）检测的实验演示。传感器表面先前经过 ssDNA 探针的生物功能化处理，可作为特定的靶受体使用。我们的实验表明，使用平面光子晶体结构可以检测到这些杂交事件，对于互补 DNA 链的检测，估计检测限低至 19.8 nM。