Krishna Veni N, Karthika D, Surya Devi M, Rubini Mf, Vishalini M, Pradeepa Yj
Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus College of J. S. S. University, Mysore), Rocklands, Ooty - 643 001, Tamilnadu, India.
J Young Pharm. 2010 Jul;2(3):297-300. doi: 10.4103/0975-1483.66795.
A simple, fast, specific, and precise high-performance thin layer chromatography method has been developed for the estimation of monosodium l-glutamate (MSG) in food products. Aluminum plates precoated with silica gel 60 GF(254)were used as stationary phase and a mixture of methanol-chloroform-formic acid in the ratio 5:5:1 (v/v) as mobile phase. Quantification was carried out by postchromatographic derivatization using 1% ninhydrin solution, and the developed spots were scanned by using a densitometer in absorbance mode at 485 nM. The R(f)value of MSG was 0.64. The results of the analysis have been validated statistically and by the recovery studies. Linearity was observed in the concentration range of 400-1000 nG.
已开发出一种简单、快速、特异且精确的高效薄层色谱法，用于食品中 l-谷氨酸钠（味精）的测定。以预涂硅胶 60 GF(254)的铝板为固定相，甲醇 - 氯仿 - 甲酸按 5:5:1（v/v）比例的混合物为流动相。采用 1%茚三酮溶液进行色谱后衍生化定量，用密度计在 485 nM 吸光度模式下扫描展开的斑点。味精的比移值（R(f)）为 0.64。分析结果已通过统计学验证和回收率研究验证。在 400 - 1000 nG 的浓度范围内观察到线性关系。
