Yi Ruokun, Feng Min, Chen Qiuping, Long Xingyao, Park Kun-Young, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
Department of Obstetrics, Eastern Hospital, Sichuan Provincial Medical Sciences Academy and Sichuan Provincial People's Hospital, Chengdu, China.
Front Nutr. 2021 May 20;8:641544. doi: 10.3389/fnut.2021.641544. eCollection 2021.
Chinese Sichuan pickle is a fermented food rich in microorganisms. Microorganisms have the potential to become an important new form of potent future therapeutic capable of treating human disease. Selecting vitamin C as a positive control, a lactic acid bacteria ( CQPC02, LP-CQPC02) isolated from Sichuan pickle was given to mice over 4 weeks to investigate the effect of CQPC02 on fatigue levels and biochemical oxidation phenomena in exercise-exhausted Institute of Cancer Research (ICR) mice. The fatigue model was established by forced swimming of mice, the levels of hepatic glycogen, skeletal muscle glycogen, lactic acid, blood urea nitrogen and free fatty acid were measured by physicochemical methods, serum serum creatine kinase (CK), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were measured by kits, the histopathological changes in the livers of mice were observed by H&E slicing, and the mRNA changes in the livers and skeletal muscles were observed by quantitative polymerase chain reaction (qPCR). Both vitamin C and LP-CQPC02 increased swimming exhaustion time. The concentration of LP-CQPC02 and exhaustion time were positively correlated. LP-CQPC02 also increased liver glycogen, skeletal muscle glycogen and free fatty acid content in mice and reduced lactic acid and blood urea nitrogen content in a dose-dependent manner. As walnut albumin antioxidant peptide concentration increased, levels of mouse CK, AST, and AST gradually decreased. LP-CQPC02 increased SOD and CAT levels and decreased MDA levels in a dose-dependent fashion. LP-CQPC02 up-regulated expression of mRNA encoding copper/zinc-superoxide dismutase (Cu/Zn-SOD), manganese-superoxide dismutase (Mn-SOD), and CAT in swimming exhaustion mouse liver tissue. LP-CQPC02 also up-regulated alanine/serine/cysteine/threonine transporter 1 (ASCT1) expression while down-regulating syncytin-1, inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-α) expression in swimming exhaustion mouse skeletal muscle. Overall, LP-CQPC02 had a clear anti-fatigue and anti-oxidation effect. This suggests that LP-CQPC02 can be developed as a microbiological therapeutic agent.
中国四川泡菜是一种富含微生物的发酵食品。微生物有可能成为未来一种重要的新型有效治疗手段，用于治疗人类疾病。以维生素C作为阳性对照，将从四川泡菜中分离出的一株乳酸菌（CQPC02，LP-CQPC02）连续4周给予小鼠，以研究CQPC02对运动性疲劳的癌症研究机构（ICR）小鼠疲劳水平和生化氧化现象的影响。通过小鼠强迫游泳建立疲劳模型，采用理化方法测定肝糖原、骨骼肌糖原、乳酸、血尿素氮和游离脂肪酸水平，用试剂盒测定血清肌酸激酶（CK）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）水平，通过苏木精-伊红（H&E）切片观察小鼠肝脏组织病理学变化，通过定量聚合酶链反应（qPCR）观察肝脏和骨骼肌中的mRNA变化。维生素C和LP-CQPC02均增加了游泳疲劳时间。LP-CQPC02的浓度与疲劳时间呈正相关。LP-CQPC02还增加了小鼠肝脏糖原、骨骼肌糖原和游离脂肪酸含量，并以剂量依赖方式降低了乳酸和血尿素氮含量。随着核桃白蛋白抗氧化肽浓度增加，小鼠CK、AST和AST水平逐渐降低。LP-CQPC02以剂量依赖方式增加SOD和CAT水平并降低MDA水平。LP-CQPC02上调了游泳疲劳小鼠肝脏组织中编码铜/锌超氧化物歧化酶（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）和CAT的mRNA表达。LP-CQPC02还上调了游泳疲劳小鼠骨骼肌中丙氨酸/丝氨酸/半胱氨酸/苏氨酸转运体1（ASCT1）的表达，同时下调了合体素-1、诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）的表达。总体而言，LP-CQPC02具有明显的抗疲劳和抗氧化作用。这表明LP-CQPC02可开发成为一种微生物治疗剂。
