Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan.
Mol Biol Rep. 2011 Apr;38(4):2285-93. doi: 10.1007/s11033-010-0360-3. Epub 2010 Nov 3.
It has been long debated whether spliceosomal introns originated in the common ancestor of eukaryotes and prokaryotes. In this study, we tested the possibility that extant introns were inherited from the common ancestor of eukaryotes and prokaryotes using in silico simulation. We first identified 21 intron positions that are shared among different families of the P-Type ATPase superfamily, some of which are known to have diverged before the separation of prokaryotes and eukaryotes. Theoretical estimates of the expected number of intron positions shared by different genes suggest that the introns at those 21 positions were inserted independently. There seems to be no intron that arose from before the diversification of the P-Type ATPase superfamily. Namely, the present introns were inserted after the separation of eukaryotes and prokaryotes.
关于核酶起源于真核生物和原核生物的共同祖先，一直存在争议。在这项研究中，我们使用计算机模拟来检验现存的核酶是否是从真核生物和原核生物的共同祖先那里遗传下来的。我们首先确定了 P 型 ATP 酶超家族的不同家族之间共有的 21 个核酶位置，其中一些核酶位置在原核生物和真核生物分离之前就已经发生了分歧。不同基因之间共享核酶位置的理论估计数表明，这 21 个位置的核酶是独立插入的。似乎没有一个核酶是在 P 型 ATP 酶超家族多样化之前产生的。也就是说，现在的核酶是在真核生物和原核生物分离之后插入的。
