Wu Lihong, Guan Haifeng, Yu Li, Chou Guixing, Wang Zhengtao
Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201210, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2099-102.
Based on the approaches of TLC identification, HPLC for assaying managiferin, and of the determination of water, total ash and acid-insoluble ash in 12 samples, collected from southwest of China, the quality standard of Gentiana rhodantha has been established. The results show the reference materia medica and mangiferin can be both well used as reference substances for TLC identification; the mass fractions of mangiferin is 0.7%-4.4% (average 2.8%), water 6.1%-8.6% (average 7.2%), total ash 3.7%-10.8% (average 6.6%) and acid-insoluble ash 0.2%-2.7% (average 1.3%). The recommended standards of quantitative indexes are that the mass fractions of mangiferin is not less than 2.0%, and the water, total ash and acid-insoluble ash are not more than 9.0%, 8.0% and 3.0% respectively.
通过对采自中国西南部的12批样品进行薄层色谱（TLC）鉴别、高效液相色谱（HPLC）法测定芒果苷含量以及水分、总灰分和酸不溶性灰分的测定，建立了滇龙胆的质量标准。结果表明，对照药材和芒果苷均可作为TLC鉴别的对照品；芒果苷的质量分数为0.7% - 4.4%（平均2.8%），水分6.1% - 8.6%（平均7.2%），总灰分3.7% - 10.8%（平均6.6%），酸不溶性灰分0.2% - 2.7%（平均1.3%）。推荐的定量指标标准为芒果苷质量分数不得少于2.0%，水分、总灰分和酸不溶性灰分分别不得超过9.0%、8.0%和3.0%。
