Pan Yu, Shen Tao, Zhang Ji, Zhao Yan-Li, Wang Yuan-Zhong, Li Wan-Yi
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China; College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China.
Biomed Chromatogr. 2015 Jan;29(1):87-96. doi: 10.1002/bmc.3243. Epub 2014 May 23.
Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC-UV-MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim-Pack XR-ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile-0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R(2) > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines.
龙胆属（龙胆科）的许多物种在世界范围内作为民间药物有着悠久的应用历史。已开发并验证了一种简单快速的超高效液相色谱-紫外-串联质谱法，用于同时测定四种民族药物（坚龙胆、红直龙胆、龙胆和美丽龙胆）中的六种指标成分（龙胆苦苷、獐牙菜苦苷、马钱子酸、獐牙菜苷、芒果苷和阿魏酸）。超高效液相色谱分析在Shim-Pack XR-ODS III（150×2.0 mm，2.2 µm）上进行。流动相由乙腈-0.1%甲酸水组成，采用梯度洗脱。四种环烯醚萜类化合物以及芒果苷的检测波长选择为242 nm，阿魏酸的检测波长设定为320 nm。质谱用于低浓度分析物的鉴定和定量。所有回归方程均显示出良好的线性关系（R²＞0.9993）。准确度和精密度均在规定范围内。色谱指纹图谱分析结合主成分分析表明，四种物种的相似度值＜0.788，而龙胆和坚龙胆的相似度值＞0.993，这为四种民族药物的利用和临床应用提供了合理依据。所建立的方法似乎是这四种民族药物质量控制的有用工具。
