黄芪总苷与人参皂苷配伍对小鼠脑缺血再灌注后基质金属蛋白酶-9和基质金属蛋白酶组织抑制因子-1的影响

[Influence of astragalosides and Panax notoginseng saponins compatibility on MMP-9 and TIMP-1 after cerebral ischemia-reperfusion in mice].

作者信息

Huang Xiaoping, Tan Hua, Chen Beiyang, Deng Changqing

机构信息

Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2187-91.

PMID:21046759

Abstract

OBJECTIVE

To investigate the effect of astragalosides (AST) and Panax notoginseng saponins (PNS) compatibility on the expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metal11oproteinase-1 (TIMP-1) after cerebral ischemia/reperfusion (I/R) injury in mice, to probe into its anti-ischemic brain injury protection mechanism.

METHOD

C57BL/6N mice were randomly divided into sham-operation group, model group, AST and PNS compatibility of high, medium and low-dose group, AST group, PNS group and edaravone group. Cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 20 min followed by 24 hours reperfusion after administration for 4 days. Pathomorphism was detected with HE staining and the expression of MMP-9 and TIMP-1 protein in brain was detected by western-blot.

RESULT

The neuronal survival rate in the drug groups was significantly higher than the control group (P < 0.01), and the effect of the-middle dose compatibility group was more obvious. Factorial analysis manifested that AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility had a synergistic interaction (P < 0.01). The expression of MMP-9 protein in the drug groups was lower than the model group significantly (P < 0.01 or P < 0.05), but the expression of TIMP-1 protein was higher than the model group significantly (P < 0.01 or P < 0.05), and the effect of the-middle dose compatibility group was more obvious, the two drugs had the stacking interaction (P < 0.05).

CONCLUSION

AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility has a synergistic effect against ischemia-reperfusion injury in mice by accommodating MMP-9/TIMP-1 probably.

摘要

目的

研究黄芪总苷（AST）与人参皂苷（PNS）配伍对小鼠脑缺血/再灌注（I/R）损伤后基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制因子-1（TIMP-1）表达的影响，探讨其抗缺血性脑损伤的保护机制。

方法

将C57BL/6N小鼠随机分为假手术组、模型组、AST与PNS配伍高、中、低剂量组、AST组、PNS组和依达拉奉组。采用双侧颈总动脉结扎20分钟，给药4天后再灌注24小时的方法制备脑缺血再灌注损伤模型。用HE染色检测病理形态学变化，用蛋白质免疫印迹法检测脑组织中MMP-9和TIMP-1蛋白的表达。

结果

各药物组神经元存活率均显著高于对照组（P<0.01），中剂量配伍组效果更明显。析因分析表明，AST 110mg·kg-1与PNS 115mg·kg-1配伍具有协同作用（P<0.01）。各药物组MMP-9蛋白表达均显著低于模型组（P<0.01或P<0.05），但TIMP-1蛋白表达均显著高于模型组（P<0.01或P<0.05），中剂量配伍组效果更明显，两药具有叠加作用（P<0.05）。