远红外处理后红麻叶提取物中类黄酮含量和酪氨酸酶抑制活性的变化。
Changes in flavonoid content and tyrosinase inhibitory activity in kenaf leaf extract after far-infrared treatment.
机构信息
R&D Center, AmorePacific Corporation, Kyounggi-do, Yongin, Republic of Korea.
出版信息
Bioorg Med Chem Lett. 2010 Dec 15;20(24):7534-6. doi: 10.1016/j.bmcl.2010.09.082. Epub 2010 Oct 1.
The tyrosinase inhibitory activity of ethanolic extract of kenaf (Hibiscus cannabinus L.) leaf was evaluated before and after subjecting it to far-infrared (FIR) irradiation. The main component of the extract was analyzed as kaempferitrin (kaempferol-3,7-O-α-dirhamnoside). Prior to FIR irradiation, no inhibitory activity of the extract was detected in a tyrosinase assay. However, after FIR irradiation for 1h at 60°C, significant tyrosinase inhibitory activity (IC(50)=3500 ppm) was observed in it. In HPLC analysis, derhamnosylation products (kaempferol, afzelin, and α-rhamnoisorobin) were detected. The inhibitory activity may be due to the existence of derhamnosylation products. This study demonstrated that FIR irradiation can be used as a convenient tool for deglycosylation of flavonoid glycoside.
远红外（FIR）辐照前后苎麻（Hibiscus cannabinus L.）叶的乙醇提取物的酪氨酸酶抑制活性进行了评估。提取物的主要成分被分析为山柰酚-3,7-O-α-二鼠李糖苷（kaempferitrin）。在 FIR 辐照之前，在酪氨酸酶测定中未检测到提取物的抑制活性。然而，在 60°C 下进行 1 小时的 FIR 辐照后，观察到显著的酪氨酸酶抑制活性（IC（50）=3500ppm）。在 HPLC 分析中，检测到去鼠李糖苷化产物（山柰酚、afzelin 和 α-鼠李糖苷异芦丁）。抑制活性可能归因于去鼠李糖苷化产物的存在。本研究表明，FIR 辐照可用作黄酮糖苷去糖基化的便捷工具。
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