Lee Sang Yeol, So Young-Jin, Shin Moon Sam, Cho Jae Youl, Lee Jongsung
Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 461-701 Gyeonggi-Do, Korea.
Department of Dermatological Health Management, College of Health Science, Eulji University, Seongna-Si, 461-713 Gyeonggi-Do, Korea.
Molecules. 2014 Mar 17;19(3):3173-80. doi: 10.3390/molecules19033173.
The crude ethyl acetate extract of the leaves of Cornus macrophylla showed antibacterial activity against Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals. Bioactivity-guided separation led to the isolation of kaempferol 3-O-α-L-rhamnopyranoside (afzelin). The structure was determined based on evaluation of its spectroscopic (UV, MS, and NMR) data. The minimum inhibitory concentration (MIC) of afzelin against Pseudomonas aeruginosa was found to be 31 µg/mL. In addition, the results indicated that a hydroxyl group at C3 of the C-ring of the flavone skeleton and the rhamnose group may act as a negative factor and an enhancing factor, respectively, in the antibacterial activities of afzelin.
大叶山茱萸叶的粗乙酸乙酯提取物对铜绿假单胞菌具有抗菌活性，铜绿假单胞菌是免疫功能低下个体患病的主要原因。生物活性导向分离导致分离出山柰酚3 - O-α-L-鼠李糖苷（杨梅苷）。基于对其光谱（紫外、质谱和核磁共振）数据的评估确定了结构。发现杨梅苷对铜绿假单胞菌的最低抑菌浓度（MIC）为31μg/mL。此外，结果表明黄酮骨架C环C3位的羟基和鼠李糖基团可能分别在杨梅苷的抗菌活性中作为负因子和增强因子起作用。
