College of Environmental Science and Engineering, Hunan University, Changsha, People's Republic of China.
Appl Biochem Biotechnol. 2011 May;164(1):103-14. doi: 10.1007/s12010-010-9118-6. Epub 2010 Oct 31.
The effects of two biosurfactants, tea saponin (TS) and rhamnolipid (RL), on the production of laccase and the degradation of phenol by P. simplicissimum were investigated in solid-state fermentation consisting of rice straw, rice bran, and sawdust. Firstly, the effects of phenol on the fermentation process were studied in the absence of surfactants. Then, a phenol concentration of 3 mg/g in the fermentation was selected for detailed research with the addition of biosurfactants. The results showed that TS and RL at different concentrations had stimulative effects on the enzyme activity of laccase. The highest laccase activities during the fermentation were enhanced by 163.7%, 68.2%, and 23.3% by TS at concentrations of 0.02%, 0.06%, and 0.10%, respectively. As a result of the enhanced laccase activity, the efficiency of phenol degradation was also improved by both biosurfactants. RL caused a significant increase of fungal biomass in the early stage of the fermentation, while TS had an inhibitory effect in the whole process. These results indicated that RL could mitigate the negative effects of phenol on fungal growth and consequently improve laccase production and phenol degradation. TS was potentially applicable to phenol-polluted solid-state fermentation.
研究了茶皂素（TS）和鼠李糖脂（RL）两种生物表面活性剂对稻草、米糠和木屑固态发酵中漆酶生产和苯酚降解的影响。首先，在没有表面活性剂的情况下研究了苯酚对发酵过程的影响。然后，选择发酵中苯酚浓度为 3mg/g，并用生物表面活性剂进行详细研究。结果表明，不同浓度的 TS 和 RL 对漆酶酶活有刺激作用。TS 浓度为 0.02%、0.06%和 0.10%时，漆酶酶活分别提高了 163.7%、68.2%和 23.3%。由于漆酶活性的提高，两种生物表面活性剂都提高了苯酚的降解效率。RL 在发酵早期显著增加了真菌生物量，而 TS 在整个过程中都有抑制作用。这些结果表明，RL 可以减轻苯酚对真菌生长的负面影响，从而提高漆酶的生产和苯酚的降解。TS 可能适用于苯酚污染的固态发酵。
