Fischell Department of Bioengineering, University of Maryland, 2330 Jeong H. Kim Engineering Building, College Park, Maryland 20742, United States.
Biomacromolecules. 2010 Dec 13;11(12):3248-55. doi: 10.1021/bm101147w. Epub 2010 Nov 8.
Polymer hydrogels containing positively charged functional groups were used to investigate the critical material and biological components of FVII activation and subsequent fibrin formation in citrated plasma. A FVIIa ELISA confirmed the ability of the polymer to induce FVII activation and provided insight into the material parameters which were influential in this activation. Experiments utilizing coagulation factor depleted and inhibited plasmas indicated that FVII, FX, FII, and FI are all vital to the process outlining the general mechanism of fibrin formation from the onset of FVII activation. Dynamic mechanical analysis and swelling experiments were used to establish a critical correlation between polymer microstructure and FVII activation.
聚合物水凝胶含有带正电荷的官能团，用于研究 FVII 激活的关键材料和生物学成分，以及随后在柠檬酸盐血浆中的纤维蛋白形成。FVIIa ELISA 证实了聚合物诱导 FVII 激活的能力，并深入了解了在这种激活中起作用的材料参数。利用凝血因子耗尽和抑制的血浆进行的实验表明，FVII、FX、FII 和 FI 对于从 FVII 激活开始概述纤维蛋白形成的一般机制的过程都是至关重要的。动态力学分析和溶胀实验用于建立聚合物微观结构与 FVII 激活之间的关键相关性。
