Institute of Chemical Industry of Forest Products, CAF, National Engineering Lab. for Biomass Chemical Utilization, Key and Open Lab. on Forest Chemical Engineering, SFA, Key Lab. of Biomass Energy and Material, Nanjing 210042, China.
Molecules. 2010 Nov 4;15(11):7923-32. doi: 10.3390/molecules15117923.
The infructescence of Platycarya strobilacea is a rich source of ellagic acid (EA) which has shown antioxidant, anticancer and antimutagen properties. Response surface methodology (RSM) was used to optimize the conditions for ultrasonic extraction of EA from infructescence of P. strobilacea. A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. The content of EA in the extracts was determined by HPLC with UV detection. Three independent variables such as ultrasonic extraction temperature (°C), liquid:solid ratio (mL/g), and ultrasonic extraction time (min) were investigated. The experimental data obtained were fitted to a quadratic equation using multiple regression analysis and also analyzed by appropriate statistical methods. The 3-D response surface and the contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimum ultrasonic extraction conditions were as follows: ultrasonic extraction temperature 70 °C, liquid:solid ratio 22.5, and ultrasonic extraction time 40 min. Under these conditions, the experimental percentage value was 1.961%, which is in close agreement with the value predicted by the model.
麻栎果实是鞣花酸（EA）的丰富来源，具有抗氧化、抗癌和抗诱变特性。本研究采用响应面法（RSM）优化超声提取麻栎果实中 EA 的条件。采用中心组合设计（CCD）进行实验设计和结果分析，以获得最佳处理参数。采用高效液相色谱法（HPLC）结合紫外检测法测定提取物中 EA 的含量。考察了超声提取温度（°C）、液固比（mL/g）和超声提取时间（min）等三个独立变量。通过多元回归分析对实验数据进行拟合，并采用适当的统计方法进行分析。应用数学模型的 3D 响应面和等高线图来确定最佳条件。最佳超声提取条件为：超声提取温度 70°C，液固比 22.5，超声提取时间 40 min。在此条件下，实验百分比值为 1.961%，与模型预测值非常吻合。
