Schneider R E, Corona E, Rosales F, Schneider F E, Rodriguez O, Pineda O
Gastroenterology and Nutrition Service, Common Diseases General Hospital of the Guatemalan Social Security Institute, Guatemala City.
Am J Clin Nutr. 1990 Feb;51(2):197-201. doi: 10.1093/ajcn/51.2.197.
In vitro studies of lactose hydrolysis in milk with 20-125 neutral lactase units (NLUs) carried out at 38.0 degrees C for 15 min with a beta-galactosidase derived from Kluyveromyces lactis (Lactaid, Lactaid Inc, Pleasantville, NJ) resulted in 85-95% of the hydrolysis observed with standard incubation conditions (24 h at 4-5 degrees C with 1000 NLU/L). Thirty-three lactose-maldigesting Guatemalan subjects, 16 children and 17 adults, were challenged with oral doses of lactose in milk (children aged less than 12 mo, 2 g/kg body wt; children aged 12-24 mo, 15 g/kg body wt; older children and adults, 18 g/kg body wt) preincubated for 20 min at 38 +/- 0.5 degrees C with 50-125 NLU Lactaid. Under these conditions the subjects consumed milk without presenting any signs of intolerance. Furthermore, their breath-hydrogen excretion showed a 91-93% reduction when compared with a similar load of milk containing nonhydrolyzed lactose.
使用源自乳酸克鲁维酵母的β-半乳糖苷酶（Lactaid，Lactaid公司，新泽西州普莱森维尔），在38.0℃下对含有20 - 125中性乳糖酶单位（NLUs）的牛奶进行乳糖水解的体外研究，反应15分钟，结果显示，与标准孵育条件（4 - 5℃下24小时，1000 NLU/L）相比，乳糖水解率达到85 - 95%。33名乳糖消化不良的危地马拉受试者，16名儿童和17名成人，口服经50 - 125 NLU Lactaid在38±0.5℃下预孵育20分钟的牛奶剂量（12个月以下儿童，2 g/kg体重；12 - 24个月儿童，15 g/kg体重；大龄儿童和成人，18 g/kg体重）。在这些条件下，受试者饮用牛奶时未出现任何不耐受迹象。此外，与饮用含有未水解乳糖的类似牛奶负荷相比，他们的呼气氢排泄量减少了91 - 93%。
