Solomons N W, Guerrero A M, Torun B
Am J Clin Nutr. 1985 Feb;41(2):209-21. doi: 10.1093/ajcn/41.2.209.
The feasibility and efficacy of adding microbial beta-galactosidase enzymes directly to milk at the time of consumption was explored in adult lactose-malabsorbers. The hydrogen breath test, and on one occasion, the rise in blood glucose, were used as indices of the completeness of intraintestinal hydrolysis and absorption of milk lactose. When added to 360 ml of cow milk containing 18 g of lactose, empirical dosages of three beta-galactosidases--one from Kluyveromyces (yeast) and two from Aspergillus (fungal)--had some effectiveness in reducing postprandial H2 excretion, although no in vivo treatment at the dosages chosen was as effective as pre-incubation of the milk in vitro. The yeast enzyme also reduced symptom frequency as compared to intact milk and enhanced postprandial rises in blood glucose. The replacement therapy with exogenous, food-grade beta-galactosidases may provide a useful intervention to reduce lactose malabsorption and milk intolerance in individuals with primary lactase deficiency.
在成年乳糖吸收不良者中，探讨了在饮用牛奶时直接添加微生物β-半乳糖苷酶的可行性和效果。采用氢呼气试验，以及在某一情况下，测定血糖升高情况，作为肠道内牛奶乳糖水解和吸收完整性的指标。当将三种β-半乳糖苷酶（一种来自克鲁维酵母（酵母），两种来自曲霉（真菌））的经验剂量添加到含有18克乳糖的360毫升牛奶中时，在减少餐后氢气排泄方面有一定效果，尽管所选择剂量的体内治疗不如牛奶体外预孵育有效。与完整牛奶相比，酵母酶还降低了症状发生率，并增强了餐后血糖升高。用外源性食品级β-半乳糖苷酶进行替代疗法可能为减少原发性乳糖酶缺乏个体的乳糖吸收不良和牛奶不耐受提供一种有用的干预措施。
