Lerebours E, N'Djitoyap Ndam C, Lavoine A, Hellot M F, Antoine J M, Colin R
Groupe de Biochimie et de Physiologie Digestive et Nutritionnelle, Rouen, France.
Am J Clin Nutr. 1989 May;49(5):823-7. doi: 10.1093/ajcn/49.5.823.
Lactase-deficient subjects absorb lactose in yogurt more effectively than lactose in other dairy products. However, as all previous studies were performed without a double-blind design and only after a single ingestion of the test product, the mechanism of this enhanced absorption remains unclear. The aims of this double-blind study were 1) to evaluate lactose absorption after prolonged ingestion of yogurt and fermented-then-pasteurized milk (FPM) and 2) to assess the modification of the lactase activity of the duodenal mucosa. In 16 lactase-deficient subjects we confirmed that yogurt enhances lactose digestion, this beneficial effect being destroyed by pasteurization. Moreover, the long-term (8 d) ingestion of either yogurt or FPM does not modify the results of hydrogen breath tests in comparison with a 24-h ingestion. The mucosal lactase (Dahlquist method) and beta-galactosidase (ONPG method) activities were not significantly modified by yogurt or FPM ingestion. These results suggest that in lactase-deficient subjects no adaptation occurs after eating yogurt or FPM and that the increased lactose absorption in yogurt must be mainly related to an intraluminal process.
乳糖酶缺乏的受试者对酸奶中乳糖的吸收比对其他乳制品中乳糖的吸收更有效。然而，由于之前所有的研究都没有采用双盲设计，且仅在单次摄入测试产品后进行，这种吸收增强的机制仍不清楚。这项双盲研究的目的是：1）评估长期摄入酸奶和先发酵后巴氏杀菌牛奶（FPM）后的乳糖吸收情况；2）评估十二指肠黏膜乳糖酶活性的变化。在16名乳糖酶缺乏的受试者中，我们证实酸奶可增强乳糖消化，而这种有益效果会因巴氏杀菌而被破坏。此外，与24小时摄入相比，长期（8天）摄入酸奶或FPM不会改变呼气氢试验的结果。摄入酸奶或FPM后，黏膜乳糖酶（达尔奎斯特法）和β-半乳糖苷酶（邻硝基苯-β-D-半乳糖苷法）的活性没有显著变化。这些结果表明，在乳糖酶缺乏的受试者中，食用酸奶或FPM后不会发生适应性变化，酸奶中乳糖吸收增加一定主要与肠腔内过程有关。
