Hoffman G E, Lee W S, Attardi B, Yann V, Fitzsimmons M D
Department of Physiology, University of Pittsburgh, Pennsylvania 15217.
Endocrinology. 1990 Mar;126(3):1736-41. doi: 10.1210/endo-126-3-1736.
Immature female rats received implants containing 17 beta-estradiol on postnatal day 28 at 0900 h, followed 24 h later by either blank capsules or progesterone. Between 1500-1600 h on the day of progesterone (or blank capsule) implantation, these rats, a group of unoperated or sham controls, and a group of estrogen-progesterone-treated immature male rats were killed and perfused, and their brains processed for immunocytochemistry of c-fos antigen and LHRH. LHRH neurons consistently expressed c-fos after estrogen-progesterone treatment in females but not males; in only one of four females examined was c-fos induced after estrogen treatment. No fos was associated with LHRH neurons in the control groups. The LHRH neurons that expressed c-fos were located in the preoptic area and anterior hypothalamus; more rostral LHRH cells did not appear stimulated. These data demonstrate that gonadal steroids, administered in a paradigm that predictably produces timed stimulation of LH release, induce c-fos in LHRH neurons. The induction of c-fos in LHRH neurons provides a potentially useful and powerful tool for studying LHRH activation at the cellular level.
未成熟雌性大鼠在出生后第28天上午9点接受含17β-雌二醇的植入物，24小时后再接受空白胶囊或孕酮处理。在植入孕酮（或空白胶囊）当天的15点至16点之间，处死这些大鼠、一组未手术或假手术对照组以及一组经雌激素-孕酮处理的未成熟雄性大鼠并进行灌注，然后对其大脑进行c-fos抗原和促性腺激素释放激素（LHRH）的免疫细胞化学处理。经雌激素-孕酮处理后，雌性大鼠的LHRH神经元持续表达c-fos，而雄性大鼠则不然；在检查的四只雌性大鼠中，只有一只在接受雌激素处理后诱导出了c-fos。对照组的LHRH神经元未发现与fos相关。表达c-fos的LHRH神经元位于视前区和下丘脑前部；更靠前的LHRH细胞似乎未受刺激。这些数据表明，以可预测地产生促黄体生成素（LH）释放定时刺激的模式给予性腺类固醇，可诱导LHRH神经元中的c-fos。LHRH神经元中c-fos的诱导为在细胞水平研究LHRH激活提供了一个潜在有用且强大的工具。
