Smith D J, Earl A J, Turner G
Department of Microbiology, University of Bristol, UK.
EMBO J. 1990 Sep;9(9):2743-50. doi: 10.1002/j.1460-2075.1990.tb07461.x.
The nucleotide sequence of the Penicillium chrysogenum Oli13 acvA gene encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase, which performs the first step in penicillin biosynthesis, has been determined. The acvA gene contains an open reading frame of 11,238 bp encoding a protein of 3746 amino acids with a predicted mol. wt of 421,073 dalton. Three domains within the protein of approximately 570 amino acids have between 38% and 43% identity with each other and share similarity with two antibiotic peptide synthetases from Bacillus brevis as well as two other enzymes capable of performing ATP-pyrophosphate exchange reactions. The acvA gene is located close to the pcbC gene encoding isopenicillin N synthetase, the enzyme for the second step of beta-lactam biosynthesis, and is transcribed in the opposite orientation to it. The intergenic region of 1107 bp from which the acvA and pcbC genes are divergently transcribed has also been sequenced.
已确定产黄青霉Oli13 acvA基因的核苷酸序列，该基因编码δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶，此酶在青霉素生物合成中催化第一步反应。acvA基因包含一个11238 bp的开放阅读框，编码一个由3746个氨基酸组成的蛋白质，预测分子量为421,073道尔顿。该蛋白质中约570个氨基酸的三个结构域彼此间具有38%至43%的同一性，并且与来自短短芽孢杆菌的两种抗生素肽合成酶以及另外两种能够进行ATP-焦磷酸交换反应的酶具有相似性。acvA基因位于编码异青霉素N合成酶（β-内酰胺生物合成第二步反应的酶）的pcbC基因附近，并且与它的转录方向相反。还对acvA和pcbC基因从其发散转录的1107 bp基因间区域进行了测序。
