HIV-1 衣壳组装和成熟的氢/氘交换分析。
Hydrogen/deuterium exchange analysis of HIV-1 capsid assembly and maturation.
机构信息
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
出版信息
Structure. 2010 Nov 10;18(11):1483-91. doi: 10.1016/j.str.2010.08.016.
Abstract
Following budding, HIV-1 virions undergo a maturation process where the Gag polyprotein in the immature virus is cleaved by the viral protease and rearranges to form the mature infectious virion. Despite the wealth of structures of isolated capsid domains and an in vitro-assembled mature lattice, models of the immature lattice do not provide an unambiguous model of capsid-molecule orientation and no structural information is available for the capsid maturation pathway. Here we have applied hydrogen/deuterium exchange mass spectrometry to immature, mature, and mutant Gag particles (CA5) blocked at the final Gag cleavage event to examine the molecular basis of capsid assembly and maturation. Capsid packing arrangements were very similar for all virions, whereas immature and CA5 virions contained an additional intermolecular interaction at the hexameric, 3-fold axis. Additionally, the N-terminal β-hairpin was observed to form as a result of capsid-SP1 cleavage rather than driving maturation as previously postulated.
摘要
在出芽之后，HIV-1 病毒经历一个成熟过程，在此过程中，不成熟病毒中的 Gag 多聚蛋白被病毒蛋白酶切割，并重新排列形成成熟的感染性病毒。尽管已经有大量的分离衣壳结构域的结构和体外组装的成熟晶格的结构，但不成熟晶格的模型并不能提供衣壳分子取向的明确模型，也没有衣壳成熟途径的结构信息。在这里，我们应用氢/氘交换质谱法研究了在最后一个 Gag 切割事件处被阻断的不成熟、成熟和突变 Gag 颗粒 (CA5)，以检查衣壳组装和成熟的分子基础。所有病毒的衣壳包装排列都非常相似，而不成熟和 CA5 病毒在六聚体的 3 倍轴处存在额外的分子间相互作用。此外，观察到 N 端 β-发夹的形成是由于衣壳-SP1 切割的结果，而不是如先前假设的那样驱动成熟。
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1
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2
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Cell. 2009 Nov 13;139(4):780-90. doi: 10.1016/j.cell.2009.10.010.
3
Structure and assembly of immature HIV.未成熟HIV的结构与组装
Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11090-5. doi: 10.1073/pnas.0903535106. Epub 2009 Jun 22.
4
X-ray structures of the hexameric building block of the HIV capsid.人类免疫缺陷病毒衣壳六聚体构建模块的X射线结构。
Cell. 2009 Jun 26;137(7):1282-92. doi: 10.1016/j.cell.2009.04.063. Epub 2009 Jun 11.
5
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6
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10
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