• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

线状免疫球蛋白A大疱性皮肤病中皮肤基底膜带抗原的鉴定及抗体的分离

Identification of the cutaneous basement membrane zone antigen and isolation of antibody in linear immunoglobulin A bullous dermatosis.

作者信息

Zone J J, Taylor T B, Kadunce D P, Meyer L J

机构信息

Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City 84132.

出版信息

J Clin Invest. 1990 Mar;85(3):812-20. doi: 10.1172/JCI114508.

DOI:10.1172/JCI114508
PMID:2107211
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC296499/
Abstract

Linear IgA bullous dermatosis (LABD) is a rare blistering skin disease characterized by basement membrane zone deposition of IgA. This study identifies a tissue antigen detected by patient serum and then isolates the autoantibody using epidermis and protein bands blotted on nitrocellulose as immunoabsorbents. Sera from 10 patients (9 with cutaneous disease and 1 with cicatrizing conjunctivitis) were evaluated. Indirect immunofluorescence revealed an IgA anti-basement membrane antibody in 6 of 10 sera with monkey esophagus substrate and 9 of 10 sera with human epidermal substrate. Immunoblotting was performed on epidermal and dermal extracts prepared from skin separated at the basement membrane zone with either sodium chloride or EDTA. Saline-separated skin expressed a 97-kD band in dermal extract alone that was recognized by 4 of 10 sera. EDTA-separated skin expressed the 97-kD band in both epidermal (4 of 10 sera) and dermal (6 of 10 sera) extract. Immunoabsorption of positive sera with epidermis purified an IgA antibody that reacted uniquely with the 97-kD band. In addition, IgA antibody bound to nitrocellulose was eluted from the 97-kD band and found to uniquely bind basement membrane zone. It is likely that the 97-kD protein identified by these techniques is responsible for basement membrane binding of IgA in LABD.

摘要

线状IgA大疱性皮肤病（LABD）是一种罕见的水疱性皮肤病，其特征为IgA在基底膜带沉积。本研究鉴定了一种可被患者血清检测到的组织抗原，然后以硝酸纤维素膜上印迹的表皮和蛋白条带作为免疫吸附剂分离自身抗体。评估了10例患者的血清（9例患有皮肤病，1例患有瘢痕性结膜炎）。间接免疫荧光显示，10份血清中有6份在猴食管底物上、9份血清在人表皮底物上出现IgA抗基底膜抗体。对用氯化钠或EDTA在基底膜带分离的皮肤制备的表皮和真皮提取物进行免疫印迹分析。用盐水分离的皮肤仅在真皮提取物中表达一条97-kD条带，10份血清中有4份可识别该条带。用EDTA分离的皮肤在表皮提取物（10份血清中有4份）和真皮提取物（10份血清中有6份）中均表达97-kD条带。用表皮对阳性血清进行免疫吸附纯化出一种IgA抗体，该抗体仅与97-kD条带发生反应。此外，从97-kD条带洗脱与硝酸纤维素膜结合的IgA抗体，发现其仅与基底膜带结合。通过这些技术鉴定的97-kD蛋白可能是LABD中IgA与基底膜结合的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/10ed4df3048f/jcinvest00069-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/5445ebde5436/jcinvest00069-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/908e09480390/jcinvest00069-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/2039172f1f3a/jcinvest00069-0209-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/570eb92e6000/jcinvest00069-0209-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/009cd9f4c0a5/jcinvest00069-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/61f14fbace0f/jcinvest00069-0210-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/5de6bf74da0d/jcinvest00069-0210-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/6b80c911cc94/jcinvest00069-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/c542f22fda2f/jcinvest00069-0211-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/3d8cc0136c37/jcinvest00069-0211-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/ae2b35011a82/jcinvest00069-0211-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/10ed4df3048f/jcinvest00069-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/5445ebde5436/jcinvest00069-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/908e09480390/jcinvest00069-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/2039172f1f3a/jcinvest00069-0209-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/570eb92e6000/jcinvest00069-0209-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/009cd9f4c0a5/jcinvest00069-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/61f14fbace0f/jcinvest00069-0210-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/5de6bf74da0d/jcinvest00069-0210-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/6b80c911cc94/jcinvest00069-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/c542f22fda2f/jcinvest00069-0211-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/3d8cc0136c37/jcinvest00069-0211-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/ae2b35011a82/jcinvest00069-0211-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d84/296499/10ed4df3048f/jcinvest00069-0212-a.jpg

相似文献

1
Identification of the cutaneous basement membrane zone antigen and isolation of antibody in linear immunoglobulin A bullous dermatosis.线状免疫球蛋白A大疱性皮肤病中皮肤基底膜带抗原的鉴定及抗体的分离
J Clin Invest. 1990 Mar;85(3):812-20. doi: 10.1172/JCI114508.
2
Identification of a basement membrane zone antigen reactive with circulating IgA antibody in ocular cicatricial pemphigoid.在眼瘢痕性类天疱疮中鉴定与循环IgA抗体反应的基底膜带抗原。
J Invest Dermatol. 1993 Oct;101(4):619-23. doi: 10.1111/1523-1747.ep12366078.
3
Two groups of bullous pemphigoid antigens are identified by affinity-purified antibodies.通过亲和纯化抗体鉴定出两组大疱性类天疱疮抗原。
J Invest Dermatol. 1990 May;94(5):611-6. doi: 10.1111/1523-1747.ep12876194.
4
IgA antibodies in chronic bullous disease of childhood react with 97 kDa basement membrane zone protein.
J Invest Dermatol. 1996 Jun;106(6):1277-80. doi: 10.1111/1523-1747.ep12348993.
5
Linear IgA bullous dermatosis with IgA antibodies exclusively directed against the 180- or 230-kDa epidermal antigens.线性IgA大疱性皮肤病，其IgA抗体仅针对180或230kDa表皮抗原。
J Invest Dermatol. 1997 Jun;108(6):854-8. doi: 10.1111/1523-1747.ep12292581.
6
Identification of a novel basement membrane antigen (p84) defined by sera with antibodies to both the epidermal and dermal side of split skin.通过含有针对分裂皮肤表皮和真皮两侧抗体的血清鉴定出一种新型基底膜抗原（p84）。
J Invest Dermatol. 1994 Feb;102(2):236-40. doi: 10.1111/1523-1747.ep12371769.
7
Antigenic specificity of antibodies from patients with linear basement membrane deposition of IgA.IgA呈线性基底膜沉积的患者体内抗体的抗原特异性。
Dermatology. 1994;189 Suppl 1:64-6. doi: 10.1159/000246933.
8
Detection of autoantibody against 97-kD antigen by immunoblot, but not by indirect immunofluorescence, in a patient with linear IgA bullous dermatosis.
J Dermatol. 1996 Oct;23(10):665-9. doi: 10.1111/j.1346-8138.1996.tb02678.x.
9
Linear IgA bullous dermatosis. Characterization of a subset of patients with concurrent IgA and IgG anti-basement membrane autoantibodies.线状IgA大疱性皮肤病。伴有IgA和IgG抗基底膜自身抗体的患者亚群的特征。
Arch Dermatol. 1995 Dec;131(12):1432-7. doi: 10.1001/archderm.131.12.1432.
10
Immunogold localization of the 97-kD antigen of linear IgA bullous dermatosis (LABD) detected with patients' sera.
J Invest Dermatol. 1994 Nov;103(5):656-9. doi: 10.1111/1523-1747.ep12398417.

引用本文的文献

1
Pemphigoid disease model systems for clinical translation.用于临床转化的类天疱疮疾病模型系统
Front Immunol. 2025 Mar 17;16:1537428. doi: 10.3389/fimmu.2025.1537428. eCollection 2025.
2
Linear IgA bullous dermatosis-a fifty year experience of Warsaw Center of bullous diseases.线状IgA大疱性皮肤病——华沙大疱性疾病中心五十年经验
Front Immunol. 2025 Jan 14;15:1478318. doi: 10.3389/fimmu.2024.1478318. eCollection 2024.
3
Anti-NC16A IgA from Patients with Linear IgA Bullous Dermatosis Induce Neutrophil-Dependent Subepidermal Blistering in Mice.

本文引用的文献

1
Small intestinal biopsies and HLA types in dermatitis herpetiformis patients with granular and linear IgA skin deposits.患有颗粒状和线状IgA皮肤沉积物的疱疹样皮炎患者的小肠活检和HLA类型。
J Invest Dermatol. 1980 Jan;74(1):9-12. doi: 10.1111/1523-1747.ep12514565.
2
Linear IgA disease in adults.
Br J Dermatol. 1982 Sep;107(3):301-16. doi: 10.1111/j.1365-2133.1982.tb00360.x.
3
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.从异质蛋白质样品的重氮化纸印迹中亲和纯化抗体。
线性 IgA 大疱性皮病患者的抗 NC16A IgA 在小鼠中诱导嗜中性粒细胞依赖性表皮下水疱形成。
J Invest Dermatol. 2024 Jan;144(1):24-32.e1. doi: 10.1016/j.jid.2023.05.027. Epub 2023 Jul 10.
4
Autoantibody Profile of a Cohort of 54 Italian Patients with Linear IgA Bullous Dermatosis: LAD-1 Denoted as a Major Auto-antigen of the Lamina Lucida Subtype.54 例意大利线性 IgA 大疱性皮病患者的自身抗体谱：LAD-1 被标记为板层素 lucida 亚类的主要自身抗原。
Acta Derm Venereol. 2020 Feb 29;100(4):adv00070. doi: 10.2340/00015555-3415.
5
Serological Diagnosis of Autoimmune Bullous Skin Diseases.自身免疫性大疱性皮肤病的血清学诊断。
Front Immunol. 2019 Aug 20;10:1974. doi: 10.3389/fimmu.2019.01974. eCollection 2019.
6
Proteases in Pemphigoid Diseases.天疱疮疾病中的蛋白酶。
Front Immunol. 2019 Jun 26;10:1454. doi: 10.3389/fimmu.2019.01454. eCollection 2019.
7
Preferential Reactivity of Dipeptidyl Peptidase-IV Inhibitor-Associated Bullous Pemphigoid Autoantibodies to the Processed Extracellular Domains of BP180.二肽基肽酶-4 抑制剂相关性大疱性类天疱疮自身抗体对 BP180 细胞外结构域的优先反应性。
Front Immunol. 2019 May 29;10:1224. doi: 10.3389/fimmu.2019.01224. eCollection 2019.
8
Drug-induced linear immunoglobulin A bullous dermatosis: A French retrospective pharmacovigilance study of 69 cases.药物诱导的线性免疫球蛋白 A 大疱性皮肤病：法国回顾性药物警戒研究 69 例。
Br J Clin Pharmacol. 2019 Mar;85(3):570-579. doi: 10.1111/bcp.13827. Epub 2019 Jan 4.
9
Vancomycin Mediates IgA Autoreactivity in Drug-Induced Linear IgA Bullous Dermatosis.万古霉素介导药物诱导的线性 IgA 大疱性皮肤病中的 IgA 自身反应性。
J Invest Dermatol. 2018 Jul;138(7):1473-1480. doi: 10.1016/j.jid.2017.12.035. Epub 2018 Feb 2.
10
IgE autoreactivity in bullous pemphigoid: eosinophils and mast cells as major targets of pathogenic immune reactants.大疱性类天疱疮中的 IgE 自身反应性：嗜酸性粒细胞和肥大细胞是致病性免疫反应物的主要靶标。
Br J Dermatol. 2017 Dec;177(6):1644-1653. doi: 10.1111/bjd.15924. Epub 2017 Nov 28.
J Biol Chem. 1981 Dec 10;256(23):11955-7.
4
Characterization of bullous pemphigoid antigen: a unique basement membrane protein of stratified squamous epithelia.大疱性类天疱疮抗原的特性：一种分层鳞状上皮独特的基底膜蛋白。
Cell. 1981 Jun;24(3):897-903. doi: 10.1016/0092-8674(81)90115-x.
5
Identification of the skin basement-membrane autoantigen in epidermolysis bullosa acquisita.获得性大疱性表皮松解症中皮肤基底膜自身抗原的鉴定。
N Engl J Med. 1984 Apr 19;310(16):1007-13. doi: 10.1056/NEJM198404193101602.
6
Differentiating anti-lamina lucida and anti-sublamina densa anti-BMZ antibodies by indirect immunofluorescence on 1.0 M sodium chloride-separated skin.通过在1.0M氯化钠分离的皮肤上进行间接免疫荧光法区分抗透明板和抗致密板下抗基底膜带抗体。
J Invest Dermatol. 1984 Feb;82(2):139-44. doi: 10.1111/1523-1747.ep12259692.
7
Identification and partial characterization of pemphigoid antigen extracted from normal human skin.从正常人皮肤中提取的类天疱疮抗原的鉴定及部分特性分析
J Invest Dermatol. 1984 Jan;82(1):108-11. doi: 10.1111/1523-1747.ep12259224.
8
Localization of basement membrane components after dermal-epidermal junction separation.
J Invest Dermatol. 1983 Aug;81(2):149-53. doi: 10.1111/1523-1747.ep12543517.
9
EDTA separation and ATPase Langerhans cell staining in the mouse epidermis.小鼠表皮中乙二胺四乙酸（EDTA）分离及三磷酸腺苷酶（ATPase）朗格汉斯细胞染色
J Invest Dermatol. 1983 Feb;80(2):104-7. doi: 10.1111/1523-1747.ep12531712.
10
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.果蝇胚胎中一种主要核被膜蛋白的两种抗原相关形式的鉴定、发育调控及对热休克的反应：利用固定在硝酸纤维素印迹上的多肽改进抗体亲和纯化方法的应用
J Cell Biol. 1984 Jul;99(1 Pt 1):20-8. doi: 10.1083/jcb.99.1.20.