Zone J J, Pazderka Smith E, Powell D, Taylor T B, Smith J B, Meyer L J
Department of Internal Medicine, Veterans Affairs Medical Center, Salt Lake City, Utah.
Dermatology. 1994;189 Suppl 1:64-6. doi: 10.1159/000246933.
We reviewed the immunoreactivity of sera binding to the epidermal side of basement membrane split skin from 13 adults and 8 children with IgA alone, 9 adults with IgA and IgG and 7 adults with IgA and ocular pemphigoid. Immunoblots were done against previously described 45-, 97-, 180- and 230-kD antigens, and reactivity was confirmed by elution of antibody from nitrocellulose and binding to the basement membrane. Ten of 13 adults and 7 of 8 children reacted with the 97-kD antigen. Sera with both IgA and IgG reacted in varying patterns and on occasion with more than 1 antigen. All 7 patients with ocular cicatricial pemphigoid reacted uniquely with a 45-kD antigen.
我们检测了13名成人和8名仅患有IgA的儿童、9名患有IgA和IgG的成人以及7名患有IgA和眼部类天疱疮的成人血清与基底膜分离皮肤表皮侧的免疫反应性。针对先前描述的45kD、97kD、180kD和230kD抗原进行免疫印迹，通过从硝酸纤维素膜上洗脱抗体并与基底膜结合来确认反应性。13名成人中的10名和8名儿童中的7名与97kD抗原发生反应。同时含有IgA和IgG的血清呈现不同的反应模式，有时会与多种抗原发生反应。所有7例眼部瘢痕性类天疱疮患者均与45kD抗原发生独特反应。
