Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Appl Environ Microbiol. 2011 Jan;77(1):338-42. doi: 10.1128/AEM.02189-10. Epub 2010 Nov 12.
Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N(2)O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.
采用单细胞分离技术获得了贫营养型脱氮细菌,包括属于希瓦氏菌属、固氮螺菌属和慢生根瘤菌属的细菌。脱氮菌种群的分类组成与以前的非培养依赖性研究评估的结果相似。这些菌株的亚硝酸盐还原酶和 N(2)O 还原酶基因的测序揭示了 16S rRNA 与脱氮功能基因系统发育之间以前未知的联系。特别是,我们鉴定了慢生根瘤菌菌株,其携带的nirS 序列以前仅在非培养依赖性研究中检测到。