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nirK-harboring denitrifiers are more responsive to denitrification- inducing conditions in rice paddy soil than nirS-harboring bacteria.nirK 型脱氮微生物比 nirS 型脱氮微生物对稻田土壤的脱氮诱导条件更敏感。
Microbes Environ. 2010;25(1):45-8. doi: 10.1264/jsme2.me09160.
2
Denitrification activity and relevant bacteria revealed by nitrite reductase gene fragments in soil of temperate mixed forest.温带混交林土壤中亚硝酸盐还原酶基因片段揭示的反硝化活性和相关细菌。
Microbes Environ. 2008;23(4):337-45. doi: 10.1264/jsme2.me08541.
3
Global sequencing: a review of current molecular data and new methods available to assess microbial diversity.全球测序:当前分子数据评估微生物多样性的新方法和新方法综述。
Microbes Environ. 2008;23(4):253-68. doi: 10.1264/jsme2.me08525.
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Identification of novel betaproteobacteria in a succinate-assimilating population in denitrifying rice paddy soil by using stable isotope probing.利用稳定同位素探测技术鉴定反硝化稻田中可同化琥珀酸盐种群中的新型β变形菌。
Microbes Environ. 2008;23(3):192-200. doi: 10.1264/jsme2.23.192.
5
Detection of denitrification genes by in situ rolling circle amplification-fluorescence in situ hybridization to link metabolic potential with identity inside bacterial cells.通过原位滚环扩增-荧光原位杂交检测反硝化基因,将代谢潜能与细菌细胞内的身份联系起来。
Environ Microbiol. 2010 Sep;12(9):2508-17. doi: 10.1111/j.1462-2920.2010.02224.x. Epub 2010 Apr 16.
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Single-cell analysis and isolation for microbiology and biotechnology: methods and applications.单细胞分析和分离在微生物学和生物技术中的方法和应用。
Appl Microbiol Biotechnol. 2010 May;86(5):1281-92. doi: 10.1007/s00253-010-2524-4. Epub 2010 Mar 23.
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Complete genomic structure of the cultivated rice endophyte Azospirillum sp. B510.栽培稻内生菌 Azospirillum sp. B510 的完整基因组结构。
DNA Res. 2010 Feb;17(1):37-50. doi: 10.1093/dnares/dsp026. Epub 2010 Jan 4.
8
Isolation of functional single cells from environments using a micromanipulator: application to study denitrifying bacteria.使用微操作器从环境中分离功能单一细胞:在研究反硝化细菌中的应用。
Appl Microbiol Biotechnol. 2010 Jan;85(4):1211-7. doi: 10.1007/s00253-009-2330-z. Epub 2009 Nov 20.
9
Microbial populations responsive to denitrification-inducing conditions in rice paddy soil, as revealed by comparative 16S rRNA gene analysis.稻田土壤中响应反硝化诱导条件的微生物种群,通过比较 16S rRNA 基因分析揭示。
Appl Environ Microbiol. 2009 Nov;75(22):7070-8. doi: 10.1128/AEM.01481-09. Epub 2009 Sep 18.
10
Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE.重新评估用于通过变性梯度凝胶电泳(DGGE)对反硝化细菌进行群落调查的靶向nirS、nirK和nosZ基因的聚合酶链式反应(PCR)引物。
FEMS Microbiol Ecol. 2004 Sep 1;49(3):401-17. doi: 10.1016/j.femsec.2004.04.011.

分离携带先前未表征功能基因序列的寡营养型反硝化菌。

Isolation of oligotrophic denitrifiers carrying previously uncharacterized functional gene sequences.

机构信息

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

出版信息

Appl Environ Microbiol. 2011 Jan;77(1):338-42. doi: 10.1128/AEM.02189-10. Epub 2010 Nov 12.

DOI:10.1128/AEM.02189-10
PMID:21075882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3019733/
Abstract

Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N(2)O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.

摘要

采用单细胞分离技术获得了贫营养型脱氮细菌,包括属于希瓦氏菌属、固氮螺菌属和慢生根瘤菌属的细菌。脱氮菌种群的分类组成与以前的非培养依赖性研究评估的结果相似。这些菌株的亚硝酸盐还原酶和 N(2)O 还原酶基因的测序揭示了 16S rRNA 与脱氮功能基因系统发育之间以前未知的联系。特别是,我们鉴定了慢生根瘤菌菌株,其携带的nirS 序列以前仅在非培养依赖性研究中检测到。