• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

反硝化细菌新种嗜氮慢生根瘤菌中铜型（NirK）和细胞色素cd型（NirS）亚硝酸还原酶基因的存在

Presence of Cu-Type (NirK) and cd-Type (NirS) Nitrite Reductase Genes in the Denitrifying Bacterium Bradyrhizobium nitroreducens sp. nov.

作者信息

Jang Jeonghwan, Ashida Naoaki, Kai Ayaaki, Isobe Kazuo, Nishizawa Tomoyasu, Otsuka Shigeto, Yokota Akira, Senoo Keishi, Ishii Satoshi

机构信息

Biotechnology Institute, University of Minnesota.

Department of Applied Biological Chemistry, The University of Tokyo.

出版信息

Microbes Environ. 2018 Sep 29;33(3):326-331. doi: 10.1264/jsme2.ME18039. Epub 2018 Aug 29.

DOI:10.1264/jsme2.ME18039
PMID:30158366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6167111/
Abstract

Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1 (=JCM 18858=KCTC 62391) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

摘要

亚硝酸还原酶是反硝化作用的关键酶。有两种类型的亚硝酸还原酶：含铜的NirK和含细胞色素cd的NirS。大多数反硝化细菌要么拥有nirK要么拥有nirS，不过据报道少数菌株同时拥有这两个基因。我们在此报告土壤反硝化细菌慢生根瘤菌属TSA1菌株中存在nirK和nirS。在反硝化条件下，nirK和nirS均被鉴定且能活跃转录。基于生理、化学分类和基因组特性，TSA1菌株（=JCM 18858=KCTC 62391）代表慢生根瘤菌属内的一个新物种，我们将其命名为反硝化慢生根瘤菌（Bradyrhizobium nitroreducens sp. nov.）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/6167111/b455f78f2488/33_326_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/6167111/32692dec5887/33_326_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/6167111/b455f78f2488/33_326_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/6167111/32692dec5887/33_326_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/6167111/b455f78f2488/33_326_2.jpg

相似文献

1
Presence of Cu-Type (NirK) and cd-Type (NirS) Nitrite Reductase Genes in the Denitrifying Bacterium Bradyrhizobium nitroreducens sp. nov.反硝化细菌新种嗜氮慢生根瘤菌中铜型（NirK）和细胞色素cd型（NirS）亚硝酸还原酶基因的存在
Microbes Environ. 2018 Sep 29;33(3):326-331. doi: 10.1264/jsme2.ME18039. Epub 2018 Aug 29.
2
Redundant roles of Bradyrhizobium oligotrophicum Cu-type (NirK) and cd1-type (NirS) nitrite reductase genes under denitrifying conditions.少营养慢生根瘤菌铜型（NirK）和cd1型（NirS）亚硝酸还原酶基因在反硝化条件下的冗余作用。
FEMS Microbiol Lett. 2018 Mar 1;365(5). doi: 10.1093/femsle/fny015.
3
Spatiotemporal Characterization of San Francisco Bay Denitrifying Communities: a Comparison of nirK and nirS Diversity and Abundance.旧金山湾反硝化群落的时空特征：nirK和nirS多样性与丰度的比较
Microb Ecol. 2017 Feb;73(2):271-284. doi: 10.1007/s00248-016-0865-y. Epub 2016 Oct 5.
4
Isolation, genetic and functional characterization of novel soil nirK-type denitrifiers.新型土壤 nirK 型反硝化菌的分离、遗传及功能特性研究。
Syst Appl Microbiol. 2010 Oct;33(6):337-47. doi: 10.1016/j.syapm.2010.06.004. Epub 2010 Aug 1.
5
Potentially Mobile Denitrification Genes Identified in sp. Strain TSH58.在 sp. 菌株 TSH58 中鉴定出具有潜在移动性的反硝化基因。
Appl Environ Microbiol. 2019 Jan 9;85(2). doi: 10.1128/AEM.02474-18. Print 2019 Jan 15.
6
Capturing Compositional Variation in Denitrifying Communities: a Multiple-Primer Approach That Includes Epsilonproteobacteria.捕捉反硝化群落中的组成变化：一种包括ε-变形菌的多重引物方法。
Appl Environ Microbiol. 2017 Mar 2;83(6). doi: 10.1128/AEM.02753-16. Print 2017 Mar 15.
7
Effect of self-alkalization on nitrite accumulation in a high-rate denitrification system: Performance, microflora and enzymatic activities.自碱化对高浓度反硝化系统中亚硝酸盐积累的影响：性能、微生物群落和酶活性。
Water Res. 2016 Jan 1;88:758-765. doi: 10.1016/j.watres.2015.11.003. Epub 2015 Nov 7.
8
nirK-harboring denitrifiers are more responsive to denitrification- inducing conditions in rice paddy soil than nirS-harboring bacteria.nirK 型脱氮微生物比 nirS 型脱氮微生物对稻田土壤的脱氮诱导条件更敏感。
Microbes Environ. 2010;25(1):45-8. doi: 10.1264/jsme2.me09160.
9
The copper-responsive regulator CsoR is indirectly involved in Bradyrhizobium diazoefficiens denitrification.铜响应调控因子 CsoR 间接参与慢生根瘤菌的反硝化作用。
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad084.
10
Analysis of nitrite reductase (nirK and nirS) genes and cultivation reveal depauperate community of denitrifying bacteria in the Black Sea suboxic zone.亚硝酸盐还原酶（nirK和nirS）基因分析及培养揭示了黑海缺氧区反硝化细菌群落匮乏。
Environ Microbiol. 2007 Jan;9(1):118-30. doi: 10.1111/j.1462-2920.2006.01121.x.

引用本文的文献

1
sp. nov., a novel rhizobial species isolated from nodules.新种，一种从根瘤中分离出的新型根瘤菌物种。
Int J Syst Evol Microbiol. 2025 Jun;75(6). doi: 10.1099/ijsem.0.006807.
2
The Effects of Heavy Metal Pollution on Soil Nitrogen Transformation and Rice Volatile Organic Compounds under Different Water Management Practices.不同水分管理措施下重金属污染对土壤氮转化及水稻挥发性有机化合物的影响
Plants (Basel). 2024 Mar 18;13(6):871. doi: 10.3390/plants13060871.
3
Phylogenetics and environmental distribution of nitric oxide-forming nitrite reductases reveal their distinct functional and ecological roles.

本文引用的文献

1
Redundant roles of Bradyrhizobium oligotrophicum Cu-type (NirK) and cd1-type (NirS) nitrite reductase genes under denitrifying conditions.少营养慢生根瘤菌铜型（NirK）和cd1型（NirS）亚硝酸还原酶基因在反硝化条件下的冗余作用。
FEMS Microbiol Lett. 2018 Mar 1;365(5). doi: 10.1093/femsle/fny015.
2
Noviherbaspirillum denitrificans sp. nov., a denitrifying bacterium isolated from rice paddy soil and Noviherbaspirillum autotrophicum sp. nov., a denitrifying, facultatively autotrophic bacterium isolated from rice paddy soil and proposal to reclassify Herbaspirillum massiliense as Noviherbaspirillum massiliense comb. nov.反硝化新草螺菌新种，一种从稻田土壤中分离出的反硝化细菌，以及自养新草螺菌新种，一种从稻田土壤中分离出的反硝化兼性自养细菌，还有将马赛草螺菌重新分类为马赛新草螺菌组合新种的提议
Int J Syst Evol Microbiol. 2017 Jun;67(6):1841-1848. doi: 10.1099/ijsem.0.001875. Epub 2017 Jun 20.
3
形成一氧化氮的亚硝酸还原酶的系统发育学与环境分布揭示了它们独特的功能和生态作用。
ISME Commun. 2024 Feb 2;4(1):ycae020. doi: 10.1093/ismeco/ycae020. eCollection 2024 Jan.
4
Diverse spp. with Similar Symbiosis Genes Nodulate Peanut in Different Regions of China: Characterization of Symbiovar sv. Arachis.具有相似共生基因的多种物种在中国不同地区使花生结瘤：共生变种花生共生体的特征分析
Plants (Basel). 2023 Nov 6;12(21):3776. doi: 10.3390/plants12213776.
5
Nitrogen fertilization promoted microbial growth and NO emissions by increasing the abundance of and denitrifiers in semiarid maize field.氮肥通过增加半干旱玉米田反硝化细菌和亚硝酸盐还原菌的丰度促进了微生物生长和一氧化氮排放。
Front Microbiol. 2023 Aug 31;14:1265562. doi: 10.3389/fmicb.2023.1265562. eCollection 2023.
6
Molecular and Dual-Isotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge.在含水层人工补给管理下，农业土壤氮淋失的微生物控制的分子和双重同位素分析。
Environ Sci Technol. 2023 Aug 1;57(30):11084-11095. doi: 10.1021/acs.est.3c01356. Epub 2023 Jul 19.
7
Impact of soil amendments on nitrous oxide emissions and the associated denitrifying communities in a semi-arid environment.土壤改良剂对半干旱环境中一氧化二氮排放及相关反硝化群落的影响
Front Microbiol. 2022 Aug 17;13:905157. doi: 10.3389/fmicb.2022.905157. eCollection 2022.
8
The Microbial Nitrogen Cycling, Bacterial Community Composition, and Functional Potential in a Natural Grassland Are Stable from Breaking Dormancy to Being Dormant Again.天然草地中从打破休眠到再次休眠期间，微生物氮循环、细菌群落组成及功能潜力保持稳定。
Microorganisms. 2022 Apr 28;10(5):923. doi: 10.3390/microorganisms10050923.
9
Distinct Community Composition of Previously Uncharacterized Denitrifying Bacteria and Fungi across Different Land-Use Types.不同土地利用类型中未经描述的反硝化细菌和真菌的独特群落组成。
Microbes Environ. 2020;35(1). doi: 10.1264/jsme2.ME19064.
10
Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for NO Reductase and Lack of the Type VI Secretion System.非豆科高粱植物中的慢生根瘤菌多样性：B. ottawaense 分离株在硝酸盐还原酶基因和缺乏 VI 型分泌系统方面具有独特性。
Microbes Environ. 2020;35(1). doi: 10.1264/jsme2.ME19102.
Genomics and Metagenomics in Microbial Ecology: Recent Advances and Challenges.微生物生态学中的基因组学和宏基因组学：最新进展与挑战
Microbes Environ. 2017;32(1):1-4. doi: 10.1264/jsme2.ME3201rh.
4
Denitrification and Nitrate-Dependent Fe(II) Oxidation in Various Pseudogulbenkiania Strains.不同假古尔本基尼亚菌株中的反硝化作用及硝酸盐依赖的亚铁氧化作用
Microbes Environ. 2016 Sep 29;31(3):293-8. doi: 10.1264/jsme2.ME16001. Epub 2016 Jul 15.
5
NCBI prokaryotic genome annotation pipeline.美国国立生物技术信息中心原核生物基因组注释管道
Nucleic Acids Res. 2016 Aug 19;44(14):6614-24. doi: 10.1093/nar/gkw569. Epub 2016 Jun 24.
6
Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis.慢生根瘤菌属豇豆根瘤菌新种，一种从豇豆属和落花生属有效根瘤中分离出的固氮共生菌。
Int J Syst Evol Microbiol. 2016 Jan;66(1):62-69. doi: 10.1099/ijsem.0.000674. Epub 2015 Oct 12.
7
Intergenomic comparisons highlight modularity of the denitrification pathway and underpin the importance of community structure for N2O emissions.基因组间的比较突出了反硝化途径的模块化，并强调了群落结构对一氧化二氮排放的重要性。
PLoS One. 2014 Dec 1;9(12):e114118. doi: 10.1371/journal.pone.0114118. eCollection 2014.
8
Bradyrhizobium ottawaense sp. nov., a symbiotic nitrogen fixing bacterium from root nodules of soybeans in Canada.加拿大大豆根瘤共生固氮菌布拉德氏菌属渥太华亚种，新种。
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3202-3207. doi: 10.1099/ijs.0.065540-0. Epub 2014 Jun 26.
9
A proposed genus boundary for the prokaryotes based on genomic insights.基于基因组见解的原核生物属界提议。
J Bacteriol. 2014 Jun;196(12):2210-5. doi: 10.1128/JB.01688-14. Epub 2014 Apr 4.
10
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.MEGA6：分子进化遗传学分析版本 6.0。
Mol Biol Evol. 2013 Dec;30(12):2725-9. doi: 10.1093/molbev/mst197. Epub 2013 Oct 16.