反硝化细菌新种嗜氮慢生根瘤菌中铜型（NirK）和细胞色素cd型（NirS）亚硝酸还原酶基因的存在
Presence of Cu-Type (NirK) and cd-Type (NirS) Nitrite Reductase Genes in the Denitrifying Bacterium Bradyrhizobium nitroreducens sp. nov.
作者信息
Jang Jeonghwan, Ashida Naoaki, Kai Ayaaki, Isobe Kazuo, Nishizawa Tomoyasu, Otsuka Shigeto, Yokota Akira, Senoo Keishi, Ishii Satoshi
机构信息
Biotechnology Institute, University of Minnesota.
Department of Applied Biological Chemistry, The University of Tokyo.
出版信息
Microbes Environ. 2018 Sep 29;33(3):326-331. doi: 10.1264/jsme2.ME18039. Epub 2018 Aug 29.
Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1 (=JCM 18858=KCTC 62391) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.
亚硝酸还原酶是反硝化作用的关键酶。有两种类型的亚硝酸还原酶：含铜的NirK和含细胞色素cd的NirS。大多数反硝化细菌要么拥有nirK要么拥有nirS，不过据报道少数菌株同时拥有这两个基因。我们在此报告土壤反硝化细菌慢生根瘤菌属TSA1菌株中存在nirK和nirS。在反硝化条件下，nirK和nirS均被鉴定且能活跃转录。基于生理、化学分类和基因组特性，TSA1菌株（=JCM 18858=KCTC 62391）代表慢生根瘤菌属内的一个新物种，我们将其命名为反硝化慢生根瘤菌（Bradyrhizobium nitroreducens sp. nov.）。
Zotero 插件