Zambrano-Nava Sunny, Boscán-Ocando Julio, Nava Jexenia
Instituto Nacional de Investigaciones Agrícolas. Estación Local Carrasquero, Unidad de Investigaciones Zootécnicas (UNIZ-FCV-LUZ), Maracaibo, Zulia, Venezuela.
Trop Anim Health Prod. 2011 Feb;43(2):291-4. doi: 10.1007/s11250-010-9701-4. Epub 2010 Nov 17.
In order to describe the normal bacterial flora in vaginas of Criollo Limonero cows, 51 healthy multiparous cows, at least 90-day postpartum, were selected. Duplicated swabs (N = 102) were taken from the vaginal fornix of cows to perform aerobic and anaerobic cultures as well as conventional biochemical tests. Out of 102 swabs, bacterial growth was obtained in 55 (53.9%) while the remaining 47 (46.1%) did not exhibited any bacterial growth. Of the 55 bacterial growths, 23 (41.8%) were aerobic whereas 32 (58.1%) were anaerobic. Likewise, 29 (52.72%) of bacterial growths were pure and 26 (47.27%) were mixed. Under both aerobic and anaerobic conditions, Gram positive bacteria were predominant (81.82% and 73.08%, respectively) over Gram negative bacteria (18.18% and 26.92%, respectively). Isolated bacteria were Arcanobacterium pyogenes (22.92%), Staphylococcus aureus (15.63%), Staphylococcus coagulase negative (17.71%), Erysipelothrix rhusiopathiae (6.25%), Bacteroides spp. (13.54%), and Peptostreptococcus spp. (7.29%). In conclusion, normal vaginal bacterial flora of Criollo Limonero cows was predominantly Gram positive and included A. pyogenes, S. aureus, coagulase negative Staphylococcus, E. rhusiopathiae, Bacteroides spp., and Peptostreptococcus spp. In Criollo Limonero cattle, adaptive aspects such as development of humoral and physical mechanisms for defense, and bacterial adaptation to host deserve research attention.
为了描述克里奥罗·利莫内罗奶牛阴道内的正常细菌菌群，选取了51头产后至少90天的健康经产奶牛。从奶牛阴道穹窿处采集双份拭子（n = 102），进行需氧和厌氧培养以及常规生化试验。在102份拭子中，55份（53.9%）有细菌生长，其余47份（46.1%）未显示任何细菌生长。在55份有细菌生长的样本中，23份（41.8%）为需氧菌，32份（58.1%）为厌氧菌。同样，29份（52.72%）细菌生长为纯培养，26份（47.27%）为混合培养。在需氧和厌氧条件下，革兰氏阳性菌均占主导地位（分别为81.82%和73.08%），高于革兰氏阴性菌（分别为18.18%和26.92%）。分离出的细菌有化脓隐秘杆菌（22.92%）、金黄色葡萄球菌（15.63%）、凝固酶阴性葡萄球菌（17.71%）、猪红斑丹毒丝菌（6.25%）、拟杆菌属（13.54%）和消化链球菌属（7.29%）。总之，克里奥罗·利莫内罗奶牛阴道内的正常细菌菌群主要为革兰氏阳性菌，包括化脓隐秘杆菌、金黄色葡萄球菌、凝固酶阴性葡萄球菌、猪红斑丹毒丝菌、拟杆菌属和消化链球菌属。在克里奥罗·利莫内罗牛中，诸如体液和物理防御机制的发育以及细菌对宿主的适应性等适应性方面值得研究关注。
