State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
ACS Nano. 2010 Dec 28;4(12):7169-74. doi: 10.1021/nn101696r. Epub 2010 Nov 17.
The exploration of efficient DNA intercalative agents (intercalators) is essential for understanding DNA scission, repair, and signal transduction. In this work, we explored systematically the graphene oxide (GO) interaction with DNA molecules using fluorescence spectroscopic (FL) and circular dichroism (CD) studies, gel electrophoresis, and DNA thermal denaturation. We demonstrated that the GO nanosheets could intercalate efficiently into DNA molecules. Significantly, we illustrated that the scission of DNA by GO sheets combining with copper ions could take place pronouncedly. The scission of DNA by the GO/Cu(2+) system is critically dependent on the concentrations of GO and Cu(2+) and their ratio. DNA cleavage ability exhibited by the GO with several other metal ions and the fact that GO/Cu(2+)-cleaved DNA fragments can be partially relegated suggest that the mechanism of DNA cleavage by the GO/metal ion system is oxidative and hydrolytic. The result reveals that the GO/Cu(2+) could be used as a DNA cleaving system that should find many practical applications in biotechnology and as therapeutic agents.
探索高效的 DNA 插入剂（嵌入剂）对于理解 DNA 的断裂、修复和信号转导至关重要。在这项工作中，我们使用荧光光谱（FL）和圆二色性（CD）研究、凝胶电泳和 DNA 热变性系统地研究了氧化石墨烯（GO）与 DNA 分子的相互作用。我们证明了 GO 纳米片可以有效地插入 DNA 分子中。重要的是，我们说明了 GO 薄片与铜离子结合可以明显地导致 DNA 的断裂。GO/Cu(2+)系统导致的 DNA 断裂严重依赖于 GO 和 Cu(2+)的浓度及其比例。GO 与其他几种金属离子的 DNA 切割能力以及 GO/Cu(2+)-切割的 DNA 片段可以部分归还的事实表明，GO/金属离子系统导致 DNA 断裂的机制是氧化和水解。结果表明，GO/Cu(2+)可作为一种 DNA 切割系统，在生物技术和治疗剂中具有广泛的应用前景。
