Trim Paul J, Francese Simona, Clench Malcolm R
Biomedical Research Centre, Sheffield Hallam University, Sheffield, Howard Street, Sheffield, S1 WB, UK.
Bioanalysis. 2009 May;1(2):309-19. doi: 10.4155/bio.09.33.
The study of drug distribution within biological tissue is a key part of the development of new pharmaceuticals. Matrix-assisted laser desorption ionization-mass spectrometric imaging is a powerful new imaging technique that can be used to study the distribution of a diverse range of endogenous and xenobiotic compounds within biological tissue. Here, fundamental aspects of the technique, appropriate instrumentation and applications in the study of xenobiotics and metabolite distribution are described. Sample preparation issues and some of the challenges in data interpretation/handling are also discussed.
生物组织内药物分布的研究是新型药物研发的关键部分。基质辅助激光解吸电离质谱成像技术是一种强大的新型成像技术，可用于研究生物组织内多种内源性和外源性化合物的分布。本文描述了该技术的基本原理、适用仪器以及在外源性物质和代谢物分布研究中的应用。还讨论了样品制备问题以及数据解释/处理中的一些挑战。
