Center of Orthognathic Surgery, Peking University School of Stomatology, Beijing, People's Republic of China.
Int J Oral Maxillofac Surg. 2011 Apr;40(4):413-8. doi: 10.1016/j.ijom.2010.10.015. Epub 2010 Nov 16.
The aim of this study was to evaluate the effect of icariin on bone formation during mandibular distraction. 40 Rabbits were randomly divided into experimental and control groups. Mandibular distraction was performed 5 days after unilateral mandibular osteotomy using a custom-made external distractor at a rate of 0.5mm/12h for 10 days. From the first day of distraction, icariin (2.5mg/kg · day) was orally administered to the experimental group and placebo to the controls. 10 Rabbits were killed at the end of weeks 2 and 4 of the consolidation phase. The distracted hemimandible was harvested and newly formed bone was evaluated by soft radiography, histology and bone histomorphometry. Regenerated bone was evaluated for bone mineral density by dual-energy X-ray absorptiometry. The experimental group had fewer radiolucent areas on soft radiography. Bone mineral density of regenerated bone was higher in the experimental than in the control group at 2 and 4 weeks. At 4 weeks, the experimental group had greater volumes of new bone, higher trabecular number, and less trabecular separation than the controls. Oral administration of icariin could promote bone formation during mandibular distraction osteogenesis and might be a promising method for shortening the course of distraction osteogenesis.
本研究旨在评估淫羊藿苷在促进下颌骨牵引成骨过程中的成骨作用。40 只兔子随机分为实验组和对照组。在单侧下颌骨切开术后 5 天，使用定制的外牵引器以 0.5mm/12h 的速度牵引 10 天。从牵引的第一天开始，实验组给予淫羊藿苷（2.5mg/kg·天），对照组给予安慰剂。在巩固期的第 2 周和第 4 周结束时，处死 10 只兔子。采集受牵引的半下颌骨，并通过软射线照相、组织学和骨组织形态计量学评估新形成的骨。双能 X 射线吸收法评估再生骨的骨矿物质密度。实验组在软射线照相上的透光区较少。实验组在第 2 周和第 4 周时，再生骨的骨矿物质密度高于对照组。在 4 周时，实验组的新骨体积更大，骨小梁数量更多，骨小梁分离度更小。淫羊藿苷的口服给药可以促进下颌骨牵引成骨过程中的骨形成，可能是缩短牵引成骨过程的一种有前途的方法。
