James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
J Gen Virol. 2011 Mar;92(Pt 3):692-8. doi: 10.1099/vir.0.026138-0. Epub 2010 Nov 17.
A papillomavirus (PV) that naturally infects laboratory mice will provide an extremely valuable tool for PV research. We describe here the isolation, cloning and molecular analysis of the first novel laboratory-mouse PV, designated MusPV. This agent, recently identified in the tissues from florid and asymmetrical papillomas on the face of nude mice (NMRI-Foxn1(nu)/Foxn1(nu)), was demonstrated to be transmissible to immunocompetent mice (Ingle et al., 2010). The MusPV genome is 7510 bp in length, is organized similarly to those of other PVs and has at least seven ORFs (E1, E2, E4, E6, E7, L1 and L2). Phylogenetic analysis indicates that MusPV belongs to the π genus together with four other rodent PVs (McPV2, MaPV1, MmiPV and RnPV1). Of the rodent PVs, MusPV appears most closely related to Mastomys coucha PV (McPV2), with 65 % genomic homogeneity and 80 % L1 amino acid similarity. Rodent PVs, except for MnPV1, do not contain any identifiable retinoblastoma protein (RB) binding sites. MusPV has one putative RB-binding site on the E6 protein but not on the E7 protein. Non-coding regions (NCRs) of PVs maintain multiple binding sites for transcription factors (TFs). The NCR of MusPV has numerous sites for TF binding, of which at least 13 TFs are common to all PVs in the π genus. MusPV provides a potentially valuable, novel mouse model to study mechanisms of infection, oncology and novel preventive and therapeutic approaches in mice that can be translated to diseases caused by human PVs.
一种能自然感染实验鼠的乳头瘤病毒（PV）将为 PV 研究提供极其有价值的工具。我们在此描述了第一个新型实验鼠 PV 的分离、克隆和分子分析，将其命名为 MusPV。这种最近在裸鼠（NMRI-Foxn1(nu)/Foxn1(nu)）面部的艳丽且不对称的乳头瘤组织中发现的病原体，已被证实可传播给免疫功能正常的小鼠（Ingle 等人，2010 年）。MusPV 基因组全长 7510bp，组织方式与其他 PV 相似，至少有七个 ORF（E1、E2、E4、E6、E7、L1 和 L2）。系统进化分析表明，MusPV 与另外四种啮齿动物 PV（McPV2、MaPV1、MmiPV 和 RnPV1）一起属于π属。在这些啮齿动物 PV 中，MusPV 与 Mastomys coucha PV（McPV2）最为接近，基因组同源性为 65%，L1 氨基酸相似性为 80%。除了 MnPV1 之外，啮齿动物 PV 都不含有任何可识别的视网膜母细胞瘤蛋白（RB）结合位点。MusPV 的 E6 蛋白上有一个假定的 RB 结合位点，但 E7 蛋白上没有。PV 的非编码区（NCR）维持着多个转录因子（TF）的结合位点。MusPV 的 NCR 有多个 TF 结合位点，其中至少有 13 个 TF 是所有 π属 PV 共有的。MusPV 为研究感染机制、肿瘤学以及可转化为人类 PV 引起的疾病的新型预防和治疗方法提供了一个有潜在价值的新型小鼠模型。
