Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC, CIBERER-Instituto de Salud Carlos III, Sevilla, Spain.
PLoS One. 2010 Nov 9;5(11):e13900. doi: 10.1371/journal.pone.0013900.
Every other day feeding (EOD) and exercise induce changes in cell metabolism. The aim of the present work was to know if both EOD and exercise produce similar effects on physical capacity, studying their physiological, biochemical and metabolic effects on muscle. Male OF-1 mice were fed either ad libitum (AL) or under EOD. After 18 weeks under EOD, animals were also trained by using a treadmill for another 6 weeks and then analyzed for physical activity. Both, EOD and endurance exercise increased the resistance of animals to extenuating activity and improved motor coordination. Among the groups that showed the highest performance, AL and EOD trained animals, ALT and EODT respectively, only the EODT group was able to increase glucose and triglycerides levels in plasma after extenuating exercise. No high effects on mitochondrial respiratory chain activities or protein levels neither on coenzyme Q levels were found in gastrocnemius muscle. However, exercise and EOD did increase β-oxidation activity in this muscle accompanied by increased CD36 levels in animals fed under EOD and by changes in shape and localization of mitochondria in muscle fibers. Furthermore, EOD and training decreased muscle damage after strenuous exercise. EOD also reduced the levels of lipid peroxidation in muscle. Our results indicate that EOD improves muscle performance and resistance by increasing lipid catabolism in muscle mitochondria at the same time that prevents lipid peroxidation and muscle damage.
隔日喂养（EOD）和运动都会引起细胞代谢的变化。本研究旨在探讨 EOD 和运动是否会产生类似的影响，从而研究它们对肌肉的生理、生化和代谢影响。雄性 OF-1 小鼠分别进行自由喂养（AL）或 EOD。在 EOD 喂养 18 周后，动物还进行了跑步机训练，持续 6 周，然后分析其体力活动。EOD 和耐力运动都提高了动物对疲劳活动的抵抗力，并改善了运动协调能力。在表现最高的组中，只有 EODT 组能够在剧烈运动后增加血浆中的葡萄糖和甘油三酯水平，而 AL 和 EOD 训练的动物（分别为 ALT 和 EODT）则不能。在比目鱼肌中，没有发现线粒体呼吸链活性或蛋白质水平以及辅酶 Q 水平的显著变化。然而，EOD 和运动确实增加了β-氧化活性，同时还增加了 EOD 喂养动物的 CD36 水平，并改变了肌肉纤维中线粒体的形状和定位。此外，EOD 和训练减少了剧烈运动后的肌肉损伤。EOD 还降低了肌肉中的脂质过氧化水平。我们的结果表明，EOD 通过增加肌肉线粒体中的脂质分解代谢来提高肌肉性能和抵抗力，同时防止脂质过氧化和肌肉损伤。
