Ferreira Sebastião R, Araújo Jackson V, Braga Fabio R, Araujo Juliana M, Carvalho Rogério O, Silva André R, Frassy Luiza N, Freitas Leandro G
Departamento de Veterinária, Universidade Federal de Viçosa, Rua PhRolfs s/n, Viçosa, Minas Gerais, Cep 36570000, Brazil.
Trop Anim Health Prod. 2011 Mar;43(3):639-42. doi: 10.1007/s11250-010-9744-6. Epub 2010 Nov 19.
The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water-agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect) among the isolates (p>0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes.
在实验室条件下测试了食线虫真菌厚垣普可尼亚菌对猪蛔虫卵的杀卵效果。将猪蛔虫卵接种在含有七种真菌分离株（分离株5、分离株31、分离株1、VC1、分离株12、分离株22和VC4）的2%水琼脂平板上，并设置无真菌的对照平板。培养5、7、10、14、15和21天后，从平板上取出约100个卵，并根据以下参数进行分类：1型，对卵壳无形态损伤的生化和生理效应；2型，卵壳和胚胎形态改变的溶解效应；3型，卵壳和胚胎形态改变且有菌丝穿透和卵内定殖的溶解效应。分离株有效地破坏了猪蛔虫卵，并且在实验过程中观察到了所有类型的效应。在整个实验过程中，各分离株之间的杀卵能力（3型效应）没有差异（p>0.05）。21天后，分离株5显示出最高的3型效应百分比（58.33%）。结果表明，厚垣普可尼亚菌（分离株5、分离株31、分离株1、VC1、分离株12、分离株22和VC4）可以破坏猪蛔虫卵，因此是一种潜在的线虫生物防治剂。
