Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
Dev Dyn. 2011 Jan;240(1):204-10. doi: 10.1002/dvdy.22491.
The expression patterns of NANOG and OCT4 have previously been reported to differ markedly between mammalian species indicating distinct species-specific roles during development. We investigate the three-dimensional expression pattern of NANOG and OCT4 in porcine pre- and peri-implantation embryos. The expression of NANOG differed remarkably from that reported in other species. NANOG was not detected in the inner cell mass of hatched porcine blastocysts, but later appeared in the epiblast and hypoblast of spherical blastocysts where Rauber's layer had disintegrated. In pre-gastrulating, filamentous embryos NANOG was localised to nuclei in a minor portion of the epiblast cells in which E-CADHERIN seemed to be up-regulated and OCT4 down-regulated. Later NANOG was restricted to the potential PGCs. OCT4 was detected in inner cell mass, epiblast, and mesoderm, and we found that OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo.
先前的研究报道表明，NANOG 和 OCT4 的表达模式在哺乳动物物种之间存在显著差异，这表明它们在发育过程中具有独特的物种特异性作用。我们研究了猪胚胎植入前和植入期胚胎中 NANOG 和 OCT4 的三维表达模式。NANOG 的表达与其他物种报道的明显不同。孵化的猪囊胚的内细胞团中未检测到 NANOG，但后来在 Rauber 层已分解的球形囊胚的上胚层和下胚层中出现。在原肠胚形成前的丝状胚胎中，NANOG 定位于一小部分上胚层细胞核中，其中 E-CADHERIN 似乎被上调，而 OCT4 被下调。后来，NANOG 局限于潜在的 PGCs。OCT4 在内细胞团、上胚层和中胚层中被检测到，我们发现 OCT4 的表达模式与之前的推测相反，至少在孵化的囊胚中，与小鼠胚胎的表达模式相似。
