Egr-1基因产物的鉴定与特性分析，一种由分化和生长信号诱导产生的DNA结合锌指蛋白。
Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.
作者信息
Cao X M, Koski R A, Gashler A, McKiernan M, Morris C F, Gaffney R, Hay R V, Sukhatme V P
机构信息
Department of Medicine, University of Chicago, Illinois 60637.
出版信息
Mol Cell Biol. 1990 May;10(5):1931-9. doi: 10.1128/mcb.10.5.1931-1939.1990.
Abstract
Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.
摘要
Egr-1是一种立即早期反应基因，由启动生长和分化的多种信号诱导产生。其cDNA序列预测出一种带有锌指结构的蛋白质。我们制备了针对Egr-1基因产物的抗血清，并在血清刺激的小鼠成纤维细胞中鉴定出它是一种80千道尔顿的短寿命蛋白质。在神经生长因子诱导的PC12细胞中也鉴定出了大鼠Egr-1产物。此外，我们通过细胞分级分离和免疫细胞化学方法表明，Egr-1蛋白位于细胞核中。我们还证明它会被磷酸化。体外产生的Egr-1蛋白以锌依赖的方式与序列CGCCCCCGC高亲和力结合。
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