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小鼠B细胞中两种κ免疫球蛋白种系转录本的起始与加工

Initiation and processing of two kappa immunoglobulin germ line transcripts in mouse B cells.

作者信息

Martin D J, van Ness B G

机构信息

Department of Biochemistry, University of Iowa, Iowa City 52242.

出版信息

Mol Cell Biol. 1990 May;10(5):1950-8. doi: 10.1128/mcb.10.5.1950-1958.1990.

DOI:10.1128/mcb.10.5.1950-1958.1990
PMID:2109186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360541/
Abstract

The splicing patterns and sequences of two processed kappa immunoglobulin germ line mRNAs are presented. A 1.1-kilobase (kb) mRNA appeared to be derived from splicing of the previously characterized 8.4-kb germ line transcript, while a 0.8-kb mRNA was the splice product of a second 4.7-kb germ line transcript that initiated 50 base pairs upstream of J kappa 1. The interaction of the two kappa germ line promoters with nuclear binding factors is also examined. The potential role of these germ line transcripts in establishing the rearrangement potential of the locus is discussed.

摘要

本文展示了两种经过加工的κ免疫球蛋白种系mRNA的剪接模式和序列。一种1.1千碱基（kb）的mRNA似乎来源于先前鉴定的8.4 kb种系转录本的剪接，而一种0.8 kb的mRNA是第二种4.7 kb种系转录本的剪接产物，该转录本在Jκ1上游50个碱基对处起始。同时也研究了两种κ种系启动子与核结合因子的相互作用。并讨论了这些种系转录本在确定该基因座重排潜能方面的潜在作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/d4f86c30eb1e/molcellb00041-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/46b6ae6b6845/molcellb00041-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/57a148607947/molcellb00041-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/d4f86c30eb1e/molcellb00041-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/46b6ae6b6845/molcellb00041-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/57a148607947/molcellb00041-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/360541/d4f86c30eb1e/molcellb00041-0127-a.jpg

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本文引用的文献

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
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DNA between variable and joining gene segments of immunoglobulin kappa light chain is frequently retained in cells that rearrange the kappa locus.免疫球蛋白κ轻链可变基因片段与连接基因片段之间的DNA经常保留在重排κ基因座的细胞中。
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RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.
Ig 内含子增强子 Eμ 在 pre-B 细胞向未成熟 B 细胞过渡中的克隆选择作用。
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Somatic generation of antibody diversity.抗体多样性的体细胞产生。
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