Department of Biological Sciences, University of Warwick, Gibbet Hill, Coventry, West Midlands CV4 7AL, United Kingdom.
Appl Environ Microbiol. 2011 Jan;77(2):684-7. doi: 10.1128/AEM.01425-10. Epub 2010 Nov 19.
The levels of integron abundance and diversity in soil amended with pig slurry were studied. Real-time PCR illustrated a significant increase in class 1 integron prevalence after slurry application, with increased prevalence still evident at 10 months after application. Culture-dependent data revealed 10 genera, including putative human pathogens, carrying class 1 and 2 integrons.
本研究旨在探讨添加猪粪对土壤中整合子丰度和多样性的影响。实时 PCR 结果表明,添加猪粪后,第 1 类整合子的流行率显著增加,且在添加后 10 个月仍保持较高水平。基于培养的数据分析显示,有 10 个属携带 1 类和 2 类整合子,其中包括可能的人类病原体。