Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK.
J Antimicrob Chemother. 2011 Jan;66(1):86-95. doi: 10.1093/jac/dkq396. Epub 2010 Nov 23.
to determine the prevalence of extended-spectrum β-lactamases (ESBLs) in Escherichia coli from poultry in Great Britain (GB).
E. coli was isolated from 388 broiler chicken caecal samples from 22 abattoirs and from boot swabs from 442 turkey flocks over successive 1 year periods. CHROMagar ECC with and without cephalosporin antibiotics was used as isolation medium and the chicken study also used CHROMagar CTX. ESBL phenotype isolates were tested for the presence of bla(CTX-M,) bla(OXA), bla(SHV), bla(TEM) and ampC genes(.) CTX-M isolates were tested for O25 serogroup, replicon, CTX-M sequence, multilocus sequence type (MLST), PFGE type, plasmid transfer and qnrA, qnrB, qnrS, qepA and aac(6')-Ib genes.
CTX-M-carrying E. coli were isolated from 54.5% of the broiler abattoirs and from 3.6% of individual broiler caecal samples and were CTX-M sequence types 1 (mainly), 3 and 15 with replicon types I1-γ, A/C and P/F, and I1-γ, respectively. CTX-M-carrying E. coli were isolated from 5.2% of turkey meat production farms and 6.9% of turkey breeder farms and were CTX-M sequence types 1, 14 (mainly), 15 and 55 with mainly replicon types F, FIA, K and I1-γ, respectively. None of the CTX-M isolates was serogroup O25. PFGE/MLST showed the CTX-M isolates to be clonally diverse, although MLST 156 with CTX-M-15 was isolated from both chickens and turkeys and has been previously reported in gulls. CTX-M-negative, ESBL- and bla(TEM)-positive strains were mainly TEM-52C.
poultry-derived CTX-M E. coli in GB are different from major CTX-M sequence types causing disease in humans.
确定英国（GB）家禽中大肠杆菌产extended-spectrum β-lactamases (ESBLs)的流行情况。
在连续的 1 年期间，从 22 家屠宰场的 388 只肉鸡盲肠样本和 442 只火鸡群的靴拭子中分离大肠杆菌。CHROMagar ECC 培养基加或不加头孢菌素抗生素被用作分离培养基，鸡的研究也使用了 CHROMagar CTX。对 ESBL 表型分离株进行 bla(CTX-M,)、bla(OXA)、bla(SHV)、bla(TEM)和 ampC 基因检测。CTX-M 分离株进行 O25 血清群、复制子、CTX-M 序列、多位点序列型（MLST）、PFGE 型、质粒转移和 qnrA、qnrB、qnrS、qepA 和 aac(6')-Ib 基因检测。
CTX-M 携带的大肠杆菌从 54.5%的肉鸡屠宰场和 3.6%的肉鸡盲肠样本中分离出来，其 CTX-M 序列类型为 1（主要）、3 和 15，分别与复制子 I1-γ、A/C 和 P/F 相关。CTX-M 携带的大肠杆菌从 5.2%的火鸡生产农场和 6.9%的火鸡饲养场中分离出来，其 CTX-M 序列类型为 1、14（主要）、15 和 55，主要与复制子 F、FIA、K 和 I1-γ 相关。CTX-M 分离株均非 O25 血清群。PFGE/MLST 显示 CTX-M 分离株具有克隆多样性，尽管 MLST 156 与 CTX-M-15 同时从鸡和火鸡中分离出来，并且之前在海鸥中报道过。CTX-M 阴性、ESBL 和 bla(TEM)阳性的菌株主要是 TEM-52C。
英国（GB）家禽来源的 CTX-M 大肠杆菌与引起人类疾病的主要 CTX-M 序列类型不同。
