对由pSV2neo转染的小鼠纤维肉瘤克隆组成的肿瘤中的克隆进化分析。
Analysis of clonal evolution in a tumor consisting of pSV2neo-transfected mouse fibrosarcoma clones.
作者信息
Enoki Y, Niwa O, Yokoro K, Toge T
机构信息
Department of Surgery, Hiroshima University.
出版信息
Jpn J Cancer Res. 1990 Feb;81(2):141-7. doi: 10.1111/j.1349-7006.1990.tb02540.x.
The process of clonal evolution was analyzed in a line of methylcholanthrene-induced mouse fibrosarcomas. The tumor cells were transfected with pSV2neo gene and 22 clones were randomly isolated. Genetically tagged clones were mixed and inoculated into syngeneic mice. Southern blot analysis revealed that one of the clones, no. 11, dominated both in tumors in situ and in lung metastatic nodules. No. 11 clone and other clones were similar in growth rates in vitro and in vivo, in spontaneous and experimental metastatic abilities, in immunogenicity, and in the capacity of intercellular communication in vitro. Although no. 11 clone overgrew other clones in vivo, this was not the case when clones were mixed and maintained in vitro. We conclude that clonal interactions in vivo may be responsible for the dominance of no. 11 clone in the tumor. It is likely that the preferential metastasis of no. 11 clone to the lung may be a simple reflection of the proliferative advantage of the dominant clone in the tumor in situ.
对一组甲基胆蒽诱导的小鼠纤维肉瘤的克隆进化过程进行了分析。用pSV2neo基因转染肿瘤细胞，并随机分离出22个克隆。将基因标记的克隆混合后接种到同基因小鼠体内。Southern印迹分析显示，其中一个克隆，即11号克隆，在原位肿瘤和肺转移结节中均占主导地位。11号克隆与其他克隆在体外和体内的生长速率、自发和实验性转移能力、免疫原性以及体外细胞间通讯能力方面相似。尽管11号克隆在体内比其他克隆生长得更快，但当克隆在体外混合培养时并非如此。我们得出结论，体内的克隆相互作用可能是11号克隆在肿瘤中占主导地位的原因。11号克隆优先转移至肺可能仅仅反映了原位肿瘤中优势克隆的增殖优势。
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