用于鉴定源自早期培养物的属于结核分枝杆菌复合群和鸟分枝杆菌复合群的热灭活分枝杆菌的酶免疫测定法。
Enzyme immunoassay for identification of heat-killed mycobacteria belonging to the Mycobacterium tuberculosis and Mycobacterium avium complexes and derived from early cultures.
作者信息
Schöningh R, Verstijnen C P, Kuijper S, Kolk A H
机构信息
N.H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 1990 Apr;28(4):708-13. doi: 10.1128/jcm.28.4.708-713.1990.
Abstract
A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis complex, two (F85-2 and F85-10) were specific for the identification of the M. avium complex, one (F126-22) was specific for M. kansasii, and one (F141-3) was broadly reactive and distinguished mycobacteria from other bacteria. In the enzyme-linked immunosorbent assay only 10(6) to 10(7) bacteria derived from early cultures (2 to 3 weeks) were needed for each monoclonal antibody. For the M. tuberculosis complex the sensitivity and specificity were both 100%. For the M. avium complex the specificity was 100% and the sensitivity was 70%. The three M. kansasii strains tested could all be identified as M. kansasii.
摘要
开发了一种简单的酶联免疫吸附测定法，用于鉴定属于结核分枝杆菌复合群、鸟分枝杆菌复合群和堪萨斯分枝杆菌的培养分枝杆菌。使用了六种单克隆抗体：两种（F23 - 49和F24 - 2）对结核分枝杆菌复合群具有特异性，两种（F85 - 2和F85 - 10）对鸟分枝杆菌复合群的鉴定具有特异性，一种（F126 - 22）对堪萨斯分枝杆菌具有特异性，一种（F141 - 3）具有广泛反应性，可将分枝杆菌与其他细菌区分开来。在酶联免疫吸附测定中，每种单克隆抗体仅需要来自早期培养物（2至3周）的10⁶至10⁷个细菌。对于结核分枝杆菌复合群，敏感性和特异性均为100%。对于鸟分枝杆菌复合群，特异性为100%，敏感性为70%。所测试的三株堪萨斯分枝杆菌均能被鉴定为堪萨斯分枝杆菌。
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