Schöningh R, Verstijnen C P, Kuijper S, Kolk A H
N.H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
J Clin Microbiol. 1990 Apr;28(4):708-13. doi: 10.1128/jcm.28.4.708-713.1990.
A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis complex, two (F85-2 and F85-10) were specific for the identification of the M. avium complex, one (F126-22) was specific for M. kansasii, and one (F141-3) was broadly reactive and distinguished mycobacteria from other bacteria. In the enzyme-linked immunosorbent assay only 10(6) to 10(7) bacteria derived from early cultures (2 to 3 weeks) were needed for each monoclonal antibody. For the M. tuberculosis complex the sensitivity and specificity were both 100%. For the M. avium complex the specificity was 100% and the sensitivity was 70%. The three M. kansasii strains tested could all be identified as M. kansasii.
开发了一种简单的酶联免疫吸附测定法，用于鉴定属于结核分枝杆菌复合群、鸟分枝杆菌复合群和堪萨斯分枝杆菌的培养分枝杆菌。使用了六种单克隆抗体：两种（F23 - 49和F24 - 2）对结核分枝杆菌复合群具有特异性，两种（F85 - 2和F85 - 10）对鸟分枝杆菌复合群的鉴定具有特异性，一种（F126 - 22）对堪萨斯分枝杆菌具有特异性，一种（F141 - 3）具有广泛反应性，可将分枝杆菌与其他细菌区分开来。在酶联免疫吸附测定中，每种单克隆抗体仅需要来自早期培养物（2至3周）的10⁶至10⁷个细菌。对于结核分枝杆菌复合群，敏感性和特异性均为100%。对于鸟分枝杆菌复合群，特异性为100%，敏感性为70%。所测试的三株堪萨斯分枝杆菌均能被鉴定为堪萨斯分枝杆菌。
