Department of Orthopaedics, Tongde Hospital of Zhejiang Province, Health Bureau of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China.
Mol Biol Rep. 2011 Jun;38(5):3569-72. doi: 10.1007/s11033-010-0467-6. Epub 2010 Nov 21.
To investigate the in vivo effect of dehydroepiandrosterone (DHEA) on the expression of aggrecanases and their endogenous inhibitor in a rabbit model of OA. Ten New Zealand white rabbits underwent bilateral anterior cruciate ligament transection (ACLT). One knee of each rabbit was randomly assigned to receive 100 μM DHEA dissolved in dimethylsulphoxide (DMSO) and the other was treated with DMSO only. The treatment was given once a week for 5 weeks, starting 4 weeks after transection. All rabbits were euthanized 9 weeks after ACLT treatment, and the knee joints were evaluated by gene expression analysis. Intra-articular administration of DHEA significantly reduced the gene expression of aggrecanases, while markly increasing that of tissue inhibitor of metalloproteinase-3 (TIMP-3), an endogenous inhibitor of aggrecanases. DHEA may have beneficial effects on OA by influencing the balance between aggrecanases and TIMP-3 through which DHEA may protect against OA.
研究脱氢表雄酮（DHEA）对兔骨关节炎模型中聚集酶及其内源性抑制剂表达的体内作用。
10 只新西兰白兔行双侧前交叉韧带切断术（ACLT）。每只兔的一侧膝关节随机接受 100μM 溶于二甲基亚砜（DMSO）的 DHEA 治疗，另一侧膝关节接受 DMSO 治疗。治疗从 ACLT 后 4 周开始，每周一次，共 5 周。所有兔子在 ACLT 治疗后 9 周处死，通过基因表达分析评估膝关节。
关节内给予 DHEA 可显著降低聚集酶的基因表达，而明显增加聚集酶内源性抑制剂金属蛋白酶组织抑制剂-3（TIMP-3）的基因表达。DHEA 可能通过影响聚集酶和 TIMP-3 之间的平衡对 OA 产生有益作用，从而起到预防 OA 的作用。
