Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan.
Acta Biomater. 2011 Apr;7(4):1558-69. doi: 10.1016/j.actbio.2010.11.018. Epub 2010 Nov 24.
Organic polymers can be uniformly surface-modified with bioactive TiO(2) by using a sol-gel method. Titania-based surface-modified polyethylene terephthalate (TiPET) plates and fabric have shown apatite-forming ability in simulated body fluid. Here, we first investigated the bone-bonding ability and mechanical bonding strength between the surface-modified layer and the base material (PET) of TiPET plates in vivo. For clinical applicability, we also examined the bone-bonding ability of TiPET fabric and the effect of titania-based surface modification on peri-implant tissue reactions (e.g. connective tissue capsule formation) in bone in vivo. Solid PET plates and PET fabric were prepared. Test plates and fabric were surface-modified with titania solution by using a sol-gel method. Histological examinations of the plates implanted into rabbit tibiae revealed direct contact between the TiPET plate and the bone. After the detaching test, a considerable amount of bone residue was observed on the surface of the TiPET plate. This result suggests that the mechanical bond strength between surface-modified layer and the base material is stronger than that between newly generated bone and tibia, and indirectly ensures the mechanical stability of the surface-modified layer. Pulling tests and histological examinations of the TiPET fabric revealed its excellent bone-bonding ability and micro-computed tomographic images showed excellent osteoconductive ability of TiPET fabric. The connective tissue capsule was much thinner, with less inflammatory tissue around the TiPET implants than around the control samples. These results indicate that TiPET fabric possesses a mechanically stable surface-modified layer, excellent bone-bonding ability, osteoconductive ability, and biocompatibility in bone.
有机聚合物可以通过溶胶-凝胶法均匀地用生物活性 TiO(2)进行表面修饰。基于钛的表面修饰的聚对苯二甲酸乙二醇酯 (TiPET) 板和织物在模拟体液中显示出形成磷灰石的能力。在这里，我们首先研究了 TiPET 板表面修饰层与基底材料 (PET) 之间的骨结合能力和机械结合强度。为了临床适用性，我们还研究了 TiPET 织物的骨结合能力以及基于钛的表面修饰对体内骨中种植体周围组织反应（例如结缔组织囊形成）的影响。制备了固体 PET 板和 PET 织物。通过溶胶-凝胶法用钛溶胶对测试板和织物进行表面修饰。将植入兔胫骨的板进行组织学检查，发现 TiPET 板与骨直接接触。在剥离试验后，在 TiPET 板表面观察到相当数量的骨残留。这一结果表明，表面修饰层与基底材料之间的机械结合强度强于新生成的骨与胫骨之间的机械结合强度，这间接保证了表面修饰层的机械稳定性。TiPET 织物的拉伸试验和组织学检查表明其具有优异的骨结合能力，微计算机断层扫描图像显示 TiPET 织物具有优异的骨引导能力。TiPET 植入物周围的结缔组织囊更薄，周围的炎症组织也比对照样品少。这些结果表明，TiPET 织物具有机械稳定的表面修饰层、优异的骨结合能力、骨引导能力和骨相容性。
