星形胶质细胞中的隧道纳米管的发育依赖于 p53 的激活。
Tunneling-nanotube development in astrocytes depends on p53 activation.
机构信息
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, China.
出版信息
Cell Death Differ. 2011 Apr;18(4):732-42. doi: 10.1038/cdd.2010.147. Epub 2010 Nov 26.
Tunneling nanotubes (TNTs) can be induced in rat hippocampal astrocytes and neurons with H(2)O(2) or serum depletion. Major cytoskeletal component of TNTs is F-actin. TNTs transfer endoplasmic reticulum, mitochondria, Golgi, endosome and intracellular as well as extracellular amyloid β. TNT development is a property of cells under stress. When two populations of cells are co-cultured, it is the stressed cells that always develop TNTs toward the unstressed cells. p53 is crucial for TNT development. When p53 function is deleted by either dominant negative construct or siRNAs, TNT development is inhibited. In addition, we find that among the genes activated by p53, epidermal growth factor receptor is also important to TNT development. Akt, phosphoinositide 3-kinase and mTOR are involved in TNT induction. Our data suggest that TNTs might be a mechanism for cells to respond to harmful signals and transfer cellular substances or energy to another cell under stress.
隧道纳米管 (TNTs) 可以在大鼠海马星形胶质细胞和神经元中通过 H₂O₂或血清耗竭诱导。TNTs 的主要细胞骨架成分是 F-肌动蛋白。TNTs 可以传递内质网、线粒体、高尔基体、内体以及细胞内和细胞外的淀粉样β。TNT 的形成是细胞在应激下的一种特性。当两种细胞群共培养时，总是应激细胞向非应激细胞形成 TNTs。p53 对 TNT 的形成至关重要。当通过显性负性构建体或 siRNA 消除 p53 的功能时，TNT 的形成就会受到抑制。此外，我们发现，在 p53 激活的基因中，表皮生长因子受体对于 TNT 的形成也很重要。Akt、磷脂酰肌醇 3-激酶和 mTOR 参与了 TNT 的诱导。我们的数据表明，TNTs 可能是细胞对有害信号做出反应并在应激下将细胞物质或能量传递到另一细胞的一种机制。
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