大鼠原代星形胶质细胞与C6胶质瘤细胞之间的隧道纳米管改变了胶质瘤细胞的增殖潜能。
Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells.
作者信息
Zhang Lei, Zhang Yan
机构信息
State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing, 100871, China.
出版信息
Neurosci Bull. 2015 Jun;31(3):371-8. doi: 10.1007/s12264-014-1522-4. Epub 2015 Apr 26.
Abstract
The tunneling nanotube (TNT) is a newly discovered, long and thin tubular structure between cells. In this study, we established a co-culture system for rat primary astrocytes and C6 glioma cells and found that TNTs formed between them. Most of the TNTs initiated from astrocytes towards C6 glioma cells. The formation of TNTs depended on p53. In addition, hydrogen peroxide increased the number of TNTs in the co-culture system. Established TNTs reduced the proliferation of C6 glioma cells. Our data suggest that TNTs between astrocytes and glioma cells facilitate substance transfer and therefore alter the properties, including the proliferation potential, of glioma cells.
摘要
隧道纳米管（TNT）是一种新发现的细胞间细长管状结构。在本研究中，我们建立了大鼠原代星形胶质细胞和C6胶质瘤细胞的共培养体系，发现它们之间形成了TNT。大多数TNT从星形胶质细胞向C6胶质瘤细胞起始。TNT的形成依赖于p53。此外，过氧化氢增加了共培养体系中TNT的数量。已形成的TNT降低了C6胶质瘤细胞的增殖。我们的数据表明，星形胶质细胞和胶质瘤细胞之间的TNT促进物质转移，从而改变胶质瘤细胞的特性，包括增殖潜能。
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本文引用的文献
1
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Commun Integr Biol. 2014 Jan 1;7(1):e27934. doi: 10.4161/cib.27934. Epub 2014 Feb 6.
2
Mesenchymal stem cells rescue injured endothelial cells in an in vitro ischemia-reperfusion model via tunneling nanotube like structure-mediated mitochondrial transfer.在体外缺血再灌注模型中，间充质干细胞通过隧道纳米管样结构介导的线粒体转移来挽救受损的内皮细胞。
Microvasc Res. 2014 Mar;92:10-8. doi: 10.1016/j.mvr.2014.01.008. Epub 2014 Jan 31.
3
Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.肿瘤外泌体诱导富含脂筏的人胸膜间皮瘤细胞中的隧道纳米管形成。
Exp Cell Res. 2014 Apr 15;323(1):178-188. doi: 10.1016/j.yexcr.2014.01.014. Epub 2014 Jan 24.
4
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Neurosci Bull. 2014 Feb;30(1):67-73. doi: 10.1007/s12264-013-1394-z. Epub 2013 Dec 13.
5
H-Ras transfers from B to T cells via tunneling nanotubes.H-Ras 通过隧道纳米管从 B 细胞转移到 T 细胞。
Cell Death Dis. 2013 Jul 18;4(7):e726. doi: 10.1038/cddis.2013.245.
6
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J Cell Sci. 2013 Aug 15;126(Pt 16):3678-85. doi: 10.1242/jcs.126086. Epub 2013 Jun 18.
7
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Hum Immunol. 2013 Apr;74(4):412-6. doi: 10.1016/j.humimm.2012.11.026. Epub 2012 Dec 7.
8
Tunneling-nanotube direction determination in neurons and astrocytes.神经元和星形胶质细胞中纳米管的隧道方向确定。
Cell Death Dis. 2012 Dec 6;3(12):e438. doi: 10.1038/cddis.2012.177.
9
Tunneling Nanotubes: A new paradigm for studying intercellular communication and therapeutics in cancer.隧道纳米管：研究癌症细胞间通讯与治疗的新范式。
Commun Integr Biol. 2012 Jul 1;5(4):399-403. doi: 10.4161/cib.20569.
10
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