Perfetto Stephen P, Ambrozak David R, Nguyen Richard, Roederer Mario, Koup Richard A, Holmes Kevin L
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2011;699:449-69. doi: 10.1007/978-1-61737-950-5_22.
Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.
在过去十年中，美国生物安全3级（BSL-3）和生物安全4级（BSL-4）实验室的数量迅速增长，BSL-3实验室对感染性细胞分选的需求也在增加。2007年，国际细胞计量学促进协会（ISAC）生物安全委员会发布了未固定细胞分选的标准，这是进行感染性细胞分选时生物安全程序的重要资源。经过仔细的风险评估，如果确定细胞分选仪必须放置在BSL-3实验室中，那么在建立实验室之前需要考虑多种因素。本章概述了在BSL-3环境中进行感染性细胞分选的程序，以促进BSL-3细胞分选实验室的建立和安全运行。涵盖的主题包括密闭性验证、远程操作、消毒、个人防护装备（PPE）以及为增强气溶胶排出而对仪器进行的特定改装。
